Pritchard A J, Chatterjee T, Wilkinson M, Powe D G, Gray T, Hewitt R E
Department of Histopathology, University of Nottingham Medical School, Queen's Medical Centre, UK.
Br J Cancer. 1995 May;71(5):1081-6. doi: 10.1038/bjc.1995.209.
Many previous qualitative studies have shown that tumours are less vascular in the centre, and that host tissues become more vascular in close proximity to tumours. However, quantitative findings presented here for human colorectal cancer reveal some significant differences. Sections from 20 colorectal carcinomas (ten moderately and ten poorly differentiated) were immunostained with the QB/end/10 monoclonal to demonstrate blood vessels. These were measured by interactive morphometry and vascular volume density, surface density (Sv) and length density were recorded. In poorly differentiated carcinomas, the tumour centre was significantly less vascular than the periphery for all three parameters (P = 0.008 for Sv). However, no significant difference was seen for moderately differentiated tumours, which constitute the majority of colorectal cancers. Surrounding host tissues did not show a general increase in vascular density close to tumours. Furthermore, when total viable tissue was considered, the vascular density of carcinomas was not markedly different from normal mucosa. In the centre of moderately differentiated carcinomas for example, the mean value for Sv was only 1.4 times higher than the mean value for normal mucosa. These findings suggest that colorectal cancers may elicit a relatively weak angiogenic response, consistent with their exceptionally slow growth rate.
许多先前的定性研究表明，肿瘤中心的血管较少，而宿主组织在靠近肿瘤处血管增多。然而，这里给出的关于人类结直肠癌的定量研究结果显示出一些显著差异。用QB/end/10单克隆抗体对20例结直肠癌（10例中度分化和10例低分化）的切片进行免疫染色以显示血管。通过交互式形态计量学对血管进行测量，并记录血管体积密度、表面密度（Sv）和长度密度。在低分化癌中，肿瘤中心在所有三个参数上的血管都明显少于周边（Sv的P值为0.008）。然而，构成大多数结直肠癌的中度分化肿瘤却未显示出显著差异。周围的宿主组织在靠近肿瘤处并未普遍显示出血管密度增加。此外，当考虑全部存活组织时，癌组织的血管密度与正常黏膜并无明显差异。例如，在中度分化癌的中心，Sv的平均值仅比正常黏膜的平均值高1.4倍。这些发现表明，结直肠癌可能引发相对较弱的血管生成反应，这与其异常缓慢的生长速度相符。
