Tsuji K, Koike K, Komiyama A, Miyajima A, Arai K, Nakahata T
Department of Clinical Oncology, University of Tokyo, Japan.
Int J Hematol. 1995 Feb;61(2):51-60. doi: 10.1016/0925-5710(95)00351-r.
We examined the effects of interleukin-10 (IL-10) on colony formation from two different types of murine mast cells, bone marrow-derived mast cells (BMMC) and serosal mast cells (SMC), using a methylcellulose culture method. IL-10 alone did not induce colony formation from either phenotype. However, BMMC and SMC produced colonies in the presence of IL-10 in combinations with IL-3, IL-4 or stem cell factor (SCF), but they responded in different manners. IL-10 enhanced the IL-3-dependent colony formation from BMMC and induced the colony formation from BMMC synergistically with IL-4 or SCF dose-dependently, although IL-4 and SCF were not active alone. The most significant synergism was observed between IL-10 and IL-4. The addition of IL-10 to the cultures of BMMC in the presence of two or three factors enhanced the colony formation induced by IL-3 plus IL-4, and inhibited the colony formation induced by IL-3 plus SCF or IL-3, IL-4 plus SCF. In the colony formation from SMC, IL-10 synergized with IL-3 but not with IL-4 or SCF. IL-10 in combination with two or three factors enhanced the colony formation from SMC induced by IL-3 plus IL-4, but did not affect the colony formation induced by other combinations among IL-3, IL-4 and SCF. These findings indicate that IL-10 plays an important role in the proliferation of murine mast cells.
我们使用甲基纤维素培养方法，研究了白细胞介素-10（IL-10）对两种不同类型小鼠肥大细胞，即骨髓来源的肥大细胞（BMMC）和浆膜肥大细胞（SMC）集落形成的影响。单独的IL-10不能诱导任何一种表型的细胞形成集落。然而，BMMC和SMC在IL-10与IL-3、IL-4或干细胞因子（SCF）联合存在的情况下能够产生集落，但它们的反应方式不同。IL-10增强了BMMC依赖IL-3的集落形成，并与IL-4或SCF协同剂量依赖性地诱导BMMC形成集落，尽管IL-4和SCF单独时没有活性。IL-10与IL-4之间观察到最显著的协同作用。在存在两种或三种因子的情况下，向BMMC培养物中添加IL-10增强了IL-3加IL-4诱导的集落形成，并抑制了IL-3加SCF或IL-3、IL-4加SCF诱导的集落形成。在SMC的集落形成中，IL-10与IL-3协同作用，但与IL-4或SCF不协同。IL-10与两种或三种因子联合增强了IL-3加IL-4诱导的SMC集落形成，但不影响IL-3、IL-4和SCF其他组合诱导的集落形成。这些发现表明IL-10在小鼠肥大细胞的增殖中起重要作用。
