Herault Y, Chatelain G, Brun G, Michel D
Laboratoire de Biologie Moléculaire et Cellulaire de l'Ecole Normale Supérieure de Lyon-CNRS UMR49, France.
Gene Expr. 1995;4(3):85-93.
The PUR element is a polypurine polypyrimidine motif that can stimulate transcription, encountered in the 5' regions of various genes and in the vicinity of several DNA replication initiation zones. We demonstrate that the PUR complex formation between the purine-rich strand of PUR and nuclear extracts can be prevented by pretreatment of nuclear extracts with RNA-damaging agents such as UV light or RNase A. A biochemical affinity method reveals that small RNA molecules copurify with the Pur factor. Moreover, the PUR binding activity of RNA-depleted nuclear extracts can be restored by addition of phenol-extracted RNAs. This work adds a new member in the emerging class of ribonucleoprotein particles as regulatory factors of the genetic expression.
PUR元件是一种多聚嘌呤-多聚嘧啶基序，可刺激转录，存在于各种基因的5'区域以及几个DNA复制起始区附近。我们证明，用紫外线或核糖核酸酶A等RNA损伤剂预处理核提取物可阻止PUR富含嘌呤链与核提取物之间形成PUR复合物。一种生化亲和方法显示，小RNA分子与Pur因子共纯化。此外，通过添加酚提取的RNA可恢复RNA去除的核提取物的PUR结合活性。这项工作在作为基因表达调控因子的核糖核蛋白颗粒这一新兴类别中增加了一个新成员。
