Pur因子的RNA依赖性DNA结合活性，可能参与DNA复制和基因转录。
RNA-dependent DNA binding activity of the Pur factor, potentially involved in DNA replication and gene transcription.
作者信息
Herault Y, Chatelain G, Brun G, Michel D
机构信息
Laboratoire de Biologie Moléculaire et Cellulaire de l'Ecole Normale Supérieure de Lyon-CNRS UMR49, France.
出版信息
Gene Expr. 1995;4(3):85-93.
Abstract
The PUR element is a polypurine polypyrimidine motif that can stimulate transcription, encountered in the 5' regions of various genes and in the vicinity of several DNA replication initiation zones. We demonstrate that the PUR complex formation between the purine-rich strand of PUR and nuclear extracts can be prevented by pretreatment of nuclear extracts with RNA-damaging agents such as UV light or RNase A. A biochemical affinity method reveals that small RNA molecules copurify with the Pur factor. Moreover, the PUR binding activity of RNA-depleted nuclear extracts can be restored by addition of phenol-extracted RNAs. This work adds a new member in the emerging class of ribonucleoprotein particles as regulatory factors of the genetic expression.
摘要
PUR元件是一种多聚嘌呤-多聚嘧啶基序，可刺激转录，存在于各种基因的5'区域以及几个DNA复制起始区附近。我们证明，用紫外线或核糖核酸酶A等RNA损伤剂预处理核提取物可阻止PUR富含嘌呤链与核提取物之间形成PUR复合物。一种生化亲和方法显示，小RNA分子与Pur因子共纯化。此外，通过添加酚提取的RNA可恢复RNA去除的核提取物的PUR结合活性。这项工作在作为基因表达调控因子的核糖核蛋白颗粒这一新兴类别中增加了一个新成员。
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本文引用的文献
1
On the role of soluble ribonucleic acid in coding for amino acids.论可溶性核糖核酸在氨基酸编码中的作用。
Proc Natl Acad Sci U S A. 1962 Jun 15;48(6):1086-92. doi: 10.1073/pnas.48.6.1086.
2
Origins of DNA replication in metazoan chromosomes.后生动物染色体中DNA复制的起源
J Biol Chem. 1993 Jan 5;268(1):1-4.
3
Analysis of a replication initiation sequence from the adenosine deaminase region of the mouse genome.对小鼠基因组腺苷脱氨酶区域复制起始序列的分析。
Mol Cell Biol. 1993 Oct;13(10):5931-42. doi: 10.1128/mcb.13.10.5931-5942.1993.
4
A unique ribonucleoprotein complex assembles preferentially on ecdysone-responsive sites in Drosophila melanogaster.一种独特的核糖核蛋白复合体优先组装在黑腹果蝇的蜕皮激素反应位点上。
Mol Cell Biol. 1993 Sep;13(9):5323-30. doi: 10.1128/mcb.13.9.5323-5330.1993.
5
Specific binding of heterogeneous ribonucleoprotein particle protein K to the human c-myc promoter, in vitro.体外实验中异质性核糖核蛋白颗粒蛋白K与人c-myc启动子的特异性结合。
J Biol Chem. 1993 Aug 25;268(24):18249-58.
6
TFIIIR is an isoleucine tRNA.TFIIIR是一种异亮氨酸转运RNA。
Mol Cell Biol. 1994 Jun;14(6):3588-95. doi: 10.1128/mcb.14.6.3588-3595.1994.
7
The PUR element stimulates transcription and is a target for single strand-specific binding factors conserved among vertebrate classes.
Cell Mol Biol Res. 1993;39(8):717-25.
8
A nuclear factor that binds purine-rich, single-stranded oligonucleotides derived from S1-sensitive elements upstream of the CFTR gene and the MUC1 gene.一种核因子，可结合源自CFTR基因和MUC1基因上游S1敏感元件的富含嘌呤的单链寡核苷酸。
Nucleic Acids Res. 1994 Apr 11;22(7):1138-46. doi: 10.1093/nar/22.7.1138.
9
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of yeast.真核转录激活蛋白——酵母GCN4的功能剖析
Cell. 1986 Sep 12;46(6):885-94. doi: 10.1016/0092-8674(86)90070-x.
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