RNA-dependent DNA binding activity of the Pur factor, potentially involved in DNA replication and gene transcription.
作者信息
Herault Y, Chatelain G, Brun G, Michel D
机构信息
Laboratoire de Biologie Moléculaire et Cellulaire de l'Ecole Normale Supérieure de Lyon-CNRS UMR49, France.
出版信息
Gene Expr. 1995;4(3):85-93.
Abstract
The PUR element is a polypurine polypyrimidine motif that can stimulate transcription, encountered in the 5' regions of various genes and in the vicinity of several DNA replication initiation zones. We demonstrate that the PUR complex formation between the purine-rich strand of PUR and nuclear extracts can be prevented by pretreatment of nuclear extracts with RNA-damaging agents such as UV light or RNase A. A biochemical affinity method reveals that small RNA molecules copurify with the Pur factor. Moreover, the PUR binding activity of RNA-depleted nuclear extracts can be restored by addition of phenol-extracted RNAs. This work adds a new member in the emerging class of ribonucleoprotein particles as regulatory factors of the genetic expression.
摘要
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本文引用的文献
1
On the role of soluble ribonucleic acid in coding for amino acids.
Proc Natl Acad Sci U S A. 1962 Jun 15;48(6):1086-92. doi: 10.1073/pnas.48.6.1086.
2
Origins of DNA replication in metazoan chromosomes.
J Biol Chem. 1993 Jan 5;268(1):1-4.
3
Analysis of a replication initiation sequence from the adenosine deaminase region of the mouse genome.
Mol Cell Biol. 1993 Oct;13(10):5931-42. doi: 10.1128/mcb.13.10.5931-5942.1993.
4
A unique ribonucleoprotein complex assembles preferentially on ecdysone-responsive sites in Drosophila melanogaster.
Mol Cell Biol. 1993 Sep;13(9):5323-30. doi: 10.1128/mcb.13.9.5323-5330.1993.
5
Specific binding of heterogeneous ribonucleoprotein particle protein K to the human c-myc promoter, in vitro.
J Biol Chem. 1993 Aug 25;268(24):18249-58.
6
TFIIIR is an isoleucine tRNA.
Mol Cell Biol. 1994 Jun;14(6):3588-95. doi: 10.1128/mcb.14.6.3588-3595.1994.
7
The PUR element stimulates transcription and is a target for single strand-specific binding factors conserved among vertebrate classes.
Cell Mol Biol Res. 1993;39(8):717-25.
8
A nuclear factor that binds purine-rich, single-stranded oligonucleotides derived from S1-sensitive elements upstream of the CFTR gene and the MUC1 gene.
Nucleic Acids Res. 1994 Apr 11;22(7):1138-46. doi: 10.1093/nar/22.7.1138.
9
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of yeast.
Cell. 1986 Sep 12;46(6):885-94. doi: 10.1016/0092-8674(86)90070-x.
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