Tanigaki-Obana N, Nakaoji K, Ito M
Hair Care Research Laboratory, Sunstar Inc., Osaka, Japan.
J Dermatol. 1995 Mar;22(3):171-4. doi: 10.1111/j.1346-8138.1995.tb03365.x.
In immunocytochemical studies of cultured hair apparatus cells we employed anti-hair keratin monoclonal antibodies (HKN-2, HKN-4, HKN-5, HKN-6 and HKN-7) and compared the results with electron microscopic studies. On day 1, the cultured cells positively stained with HKN-2 (52%), HKN-4 (50%), HKN-5 (43%), HKN-6 (33%), or HKN-7 (37%). On day 3, more than 88% of the cells showed no reaction to any antibody. On day 6, most of the cells stained with all of the antibodies: HKN-2 (96%), HKN-4 (92%), HKN-5 (80%), HKN-6 (72%) and HKN-7 (74%). These reactivities were maintained until day 13. Electron microscopic studies revealed that, on day 1, half of the observed cells were immature and 47% of them were already differentiated. Most (88%) of the cells showed immature features and the percentage of differentiated cells had decreased by day 3. The differentiated cells (87%) reappeared by day 6, and degenerated cells (63%) increased by day 13. These immunocytochemical results were consistent with those of simultaneous electron microscopic studies, demonstrating that immature cells proliferated and differentiated into subpopulations of each hair apparatus layer type, including the outer root sheath cells, in this culture system.
在对培养的毛发生成器官细胞进行免疫细胞化学研究时，我们使用了抗毛发角蛋白单克隆抗体（HKN - 2、HKN - 4、HKN - 5、HKN - 6和HKN - 7），并将结果与电子显微镜研究结果进行了比较。第1天，培养的细胞用HKN - 2（52%）、HKN - 4（50%）、HKN - 5（43%）、HKN - 6（33%）或HKN - 7（37%）呈阳性染色。第3天，超过88%的细胞对任何抗体均无反应。第6天，大多数细胞对所有抗体均呈染色阳性：HKN - 2（96%）、HKN - 4（92%）、HKN - 5（80%）、HKN - 6（72%）和HKN - 7（74%）。这些反应性一直维持到第13天。电子显微镜研究显示，第1天，观察到的细胞中有一半未成熟，其中47%已经分化。大多数（88%）细胞呈现未成熟特征，到第3天分化细胞的比例下降。到第6天，分化细胞（87%）再次出现，到第13天退化细胞（63%）增加。这些免疫细胞化学结果与同时进行的电子显微镜研究结果一致，表明在该培养系统中，未成熟细胞增殖并分化为每种毛发生成器官层类型的亚群，包括外根鞘细胞。
