Lin C, Stahl D A
Department of Veterinary Pathobiology, University of Illinois at Urbana-Champaign 61801, USA.
Appl Environ Microbiol. 1995 Apr;61(4):1348-51. doi: 10.1128/aem.61.4.1348-1351.1995.
A total of six 16S rRNA targeted oligonucleotide probes were used to quantify Fibrobacter abundance and diversity in the gastrointestinal contents of a pony. Approximately 12% of the total 16S rRNA extracted from cecal contents hybridized with a Fibrobacter genus-specific probe and a Fibrobacter succinogenes species-specific probe. However, no significant hybridization was observed with a probe for the species. Fibrobacter intestinalis or with three probes for F. succinogenes subspecies. This suggested the presence of a previously undescribed population of F. succinogenes-like organisms. Novel lineages of F. succinogenes were subsequently identified by using PCR primers specific for the genus to amplify sequences coding for 16S rRNA from DNA extracted from cecal contents. Sequences of the cloned amplification products were shown to be affiliated with F. succinogenes but represented two distinct, and novel, lines of descent within the species.
总共使用了六种靶向16S rRNA的寡核苷酸探针来量化一匹小马胃肠道内容物中纤维杆菌属的丰度和多样性。从盲肠内容物中提取的总16S rRNA中，约12%与纤维杆菌属特异性探针和琥珀酸纤维杆菌种特异性探针杂交。然而，未观察到与肠纤维杆菌种探针或三种琥珀酸纤维杆菌亚种探针的显著杂交。这表明存在一种以前未描述的类琥珀酸纤维杆菌菌群。随后，通过使用针对该属的PCR引物从盲肠内容物中提取的DNA扩增编码16S rRNA的序列，鉴定出了琥珀酸纤维杆菌的新谱系。克隆扩增产物的序列显示与琥珀酸纤维杆菌相关，但代表了该物种内两个不同的新谱系。
