Migliaccio A R, Migliaccio G, Adamson J W
Laboratory of Hematopoietic Growth Factors, New York Blood Center, NY 10021, USA.
Blood Cells. 1994;20(2-3):424-8; discussion 428-9.
Over time CD34+ cells purified from human cord blood generate large numbers of progenitor and precursor cells in liquid culture under serum-deprived conditions if stimulated with a cocktail of growth factors which include stem cell factor (SCF). The ex vivo expansion observed in liquid cultures is not homogeneous over time but involves the recruitment of different cell compartments and can be triggered by different growth factor combinations. We have recognized at least three phases in these liquid cultures. Phase I spans the first 20 days of culture. In this phase, progenitor and precursor cells are generated from the progenitor cell compartment itself in response to SCF in combination with either IL-3, erythropoietin, or G-CSF. Phase II spans the second month of culture and involves the recruitment of less and less differentiated cells by IL-3 and SCF. Phase III spans from the third month on and results in the indefinite proliferation of human mast cells. These results raise caution on the biological equivalence of liquid culture en vivo expanded hematopoietic cells at different time points.
随着时间的推移，如果用包括干细胞因子（SCF）在内的生长因子混合物刺激，从人脐带血中纯化的CD34+细胞在血清剥夺条件下的液体培养中会产生大量祖细胞和前体细胞。在液体培养中观察到的体外扩增随时间并不均匀，而是涉及不同细胞区室的募集，并且可以由不同的生长因子组合触发。我们已经认识到这些液体培养中至少有三个阶段。第一阶段跨越培养的前20天。在这个阶段，祖细胞和前体细胞是由祖细胞区室自身响应SCF与IL-3、促红细胞生成素或G-CSF的组合而产生的。第二阶段跨越培养的第二个月，涉及IL-3和SCF募集分化程度越来越低的细胞。第三阶段从第三个月开始，导致人肥大细胞的无限增殖。这些结果对不同时间点液体培养体内扩增的造血细胞的生物学等效性提出了警示。
