Rieck R W, Nabors C C, Updyke B V, Kratz K E
Department of Anatomy, Louisiana State University Medical School, New Orleans 70112, USA.
Brain Res. 1995 Feb 20;672(1-2):237-50. doi: 10.1016/0006-8993(94)01367-q.
The present study uses immunocytochemical techniques to determine whether cholinergic basal forebrain neurons in the cat are in a position to receive a homogeneous pattern of inputs, or if specific immunocytochemically defined afferent systems are localized to only selected regions of the basal forebrain. Monoclonal antibodies against choline acetyltransferase (ChAT) were used to identify the location of putative cholinergic neurons which are known to project to the cerebral cortex. In addition, polyclonal antibodies against substance P (SP) or enkephalin (Enk) were used on either adjacent or on the same histological sections reacted for ChAT to identify the neuropeptide plexuses that provide input to the basal forebrain. ChAT-immunoreactive (ChAT-IR) perikarya were located throughout the vertical limb, genu and horizontal limb of the diagonal band of Broca. ChAT-IR neurons also were located within the substantia innominata (SI), within the peripallidal zone around the globus pallidus, and were intercalated within the internal capsule. Enk-IR and SP-IR were used to determine the distribution of putative peptidergic terminals within the basal forebrain. Extensive Enk-IR and SP-IR terminal label was localized within the globus pallidus and the surrounding peripallidal zones, as well as within the SI, whereas the components of the diagonal band of Broca demonstrated negligible Enk-IR and SP-IR label. These data predict that the subdivisions of the cholinergic basal forebrain in the cat do not share a uniform afferent system, and only selective portions of this cholinergic system are in an anatomical position to receive a major direct input from the identified subcortical peptidergic afferents. The segregation of afferents has important consequences in the selective control of cortical function by the cholinergic basalocortical pathway.
本研究采用免疫细胞化学技术来确定猫的胆碱能基底前脑神经元是否能够接收均匀的输入模式，或者特定的免疫细胞化学定义的传入系统是否仅定位于基底前脑的特定区域。针对胆碱乙酰转移酶（ChAT）的单克隆抗体用于识别已知投射到大脑皮层的假定胆碱能神经元的位置。此外，针对P物质（SP）或脑啡肽（Enk）的多克隆抗体用于在与ChAT反应的相邻或同一组织学切片上，以识别向基底前脑提供输入的神经肽丛。ChAT免疫反应性（ChAT-IR）胞体位于整个布罗卡斜带的垂直支、膝部和水平支。ChAT-IR神经元也位于无名质（SI）内、苍白球周围的苍白球周区，并插入内囊。Enk-IR和SP-IR用于确定基底前脑内假定的肽能终末的分布。广泛的Enk-IR和SP-IR终末标记定位于苍白球及其周围的苍白球周区以及SI内，而布罗卡斜带的各部分显示出可忽略不计的Enk-IR和SP-IR标记。这些数据预测，猫胆碱能基底前脑的各亚区不共享统一的传入系统，并且该胆碱能系统只有选择性部分在解剖学位置上能够接收来自已确定的皮层下肽能传入纤维的主要直接输入。传入纤维的分离对胆碱能基底皮层通路对皮层功能的选择性控制具有重要影响。
