小细胞质RNA的缺失赋予枯草芽孢杆菌对夫西地酸的抗性。
Depletion of small cytoplasmic RNA confers fusidic-acid resistance on Bacillus subtilis.
作者信息
Shibata T, Nakamura Y, Nakamura K, Yamane K
机构信息
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
出版信息
Biochem Biophys Res Commun. 1995 May 16;210(2):317-23. doi: 10.1006/bbrc.1995.1663.
Bacillus subtilis small cytoplasmic RNA (scRNA) is a member of a signal recognition particle (SRP)-like RNA family. To analyze the function of scRNA in protein synthesis, a B. subtilis strain SC201NA was constructed in which the expression of intact scRNA is regulated by an IPTG-inducible promoter. In this strain, depletion of scRNA leads to deficient translation and sporulation as well as morphological changes. In addition, the growth of SC201NA in the absence of IPTG became fusidic-acid resistant. The acquisition of fusidic-acid resistant phenotype by depletion of scRNA suggested that scRNA is associated with elongation factor G (EF-G) in the translation process.
枯草芽孢杆菌小细胞质RNA（scRNA）是信号识别颗粒（SRP）样RNA家族的成员。为了分析scRNA在蛋白质合成中的功能，构建了枯草芽孢杆菌菌株SC201NA，其中完整scRNA的表达由IPTG诱导型启动子调控。在该菌株中，scRNA的缺失导致翻译和芽孢形成缺陷以及形态变化。此外，SC201NA在没有IPTG的情况下生长时对夫西地酸产生抗性。通过缺失scRNA获得夫西地酸抗性表型表明，scRNA在翻译过程中与延伸因子G（EF-G）相关。
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