Rousselet M C, Laniece A, Gardais J, Dautel M, Gardembas-Pain M, Pellier I, Ifrah N, Saint-André J P
Laboratoire d'Anatomie Pathologique, CHU, Angers.
Ann Pathol. 1995;15(2):119-26.
An immunohistochemical study by avidin-biotin-peroxidase was performed on paraffin-embedded and decalcified bone marrow biopsies in 31 acute leukemias (19 myeloid and 12 lymphoblastic). The Ulex Europaeus lectin and 14 antibodies (anti-CD45, -CD34, -myeloperoxidase, -lysozyme, -CD15, -CD68, -carcinoembryonic antigen, -factor VIII-related antigen, BNH9, anti-CD45RO, -CD3, -CD20, DBB42 and DBA44) were tested. All acute myeloid leukemias from M0 to M5 type were stained by either the anti-myeloperoxidase or anti-lysozyme antibodies. CD68, CD15 and the carcinoembryonic antigen were respectively expressed in 80%, 40% and 20% of myeloid leukemias from M1 to M5 type. The Ulex Europaeus lectin and the anti-factor VIII-related antigen antibody stained only the M7 leukemia and the anti-CD3 antibody stained only the T acute lymphoblastic leukemia. DBB42 was expressed by 63% of B-lineage lymphoblastic leukemias and CD20 by 36%. No leukemia was stained by DBA44. Immunohistochemistry on bone marrow biopsy can assess the lineage of most acute leukemias with the use of a panel of antibodies such as the anti-myeloperoxidase, -lysozyme, -CD68, -CD20, DBB42, -CD3, BNH9, anti-factor VIII-related antigen antibodies and the Ulex Europaeus lectin.
采用抗生物素蛋白-生物素-过氧化物酶免疫组化法，对31例急性白血病（19例髓系白血病和12例淋巴细胞白血病）的石蜡包埋及脱钙骨髓活检标本进行检测。检测了欧洲荆豆凝集素及14种抗体（抗CD45、抗CD34、抗髓过氧化物酶、抗溶菌酶、抗CD15、抗CD68、癌胚抗原、因子VIII相关抗原、BNH9、抗CD45RO、抗CD3、抗CD20、DBB42和DBA44）。从M0到M5型的所有急性髓系白血病均被抗髓过氧化物酶或抗溶菌酶抗体染色。CD68、CD15和癌胚抗原分别在M1到M5型髓系白血病的80%、40%和20%中表达。欧洲荆豆凝集素和抗因子VIII相关抗原抗体仅对M7白血病染色，抗CD3抗体仅对T急性淋巴细胞白血病染色。63%的B系淋巴细胞白血病表达DBB42，36%表达CD20。没有白血病被DBA44染色。骨髓活检的免疫组化可通过使用一组抗体，如抗髓过氧化物酶、抗溶菌酶、抗CD68、抗CD20、DBB42、抗CD3、BNH9、抗因子VIII相关抗原抗体和欧洲荆豆凝集素，来评估大多数急性白血病的细胞系。
