Berger T, Müller T, Kettenmann H
Institute of Anatomy, University of Freiburg, Germany.
Perspect Dev Neurobiol. 1995;2(4):347-56.
The electrophysiological properties of glial precursor cells and oligodendrocytes were studied in an intact tissue preparation, the corpus callosum slice, with the patch-clamp technique. The pattern of voltage-gated currents exhibited by different cell types in this white matter tract was compared with that from cultured cells of the O-2A lineage. Precursor cells from the in vitro and the in situ preparation were strikingly similar in most aspects. In contrast, oligodendrocytes in the intact tissue differed from their culture correlates and displayed the ability to redistribute excess K+. To study glial transmitter receptors in neuronglial interactions during development, Bergmann glial cells were investigated in a slice preparation of the cerebellum.
采用膜片钳技术，在完整的组织标本胼胝体切片中研究了神经胶质前体细胞和少突胶质细胞的电生理特性。将该白质束中不同细胞类型所表现出的电压门控电流模式与O-2A谱系培养细胞的模式进行了比较。体外和原位制备的前体细胞在大多数方面惊人地相似。相比之下，完整组织中的少突胶质细胞与其培养对应物不同，表现出重新分配过量钾离子的能力。为了研究发育过程中神经元-神经胶质相互作用中的神经胶质递质受体，在小脑切片标本中研究了伯格曼神经胶质细胞。
