Abeyama K, Kawano K, Nakajima T, Takasaki I, Kitajima I, Maruyama I
Department of Laboratory Medicine, Faculty of Medicine, Kagoshima University, Japan.
FEBS Lett. 1995 May 15;364(3):298-300. doi: 10.1016/0014-5793(95)00412-3.
The differentiation of PC12 cells to a neuron-like morphology was induced by ionizing radiation in the presence of serum. This effect was detectable at 5 grays (Gy) and reached a maximum at 10-20 Gy. Increases in the DNA binding activity of nuclear factor kappa B (NF-kappa B) and increased Interleukin 6 (IL-6) mRNA levels were observed at a dose of 15 Gy. Neutralization of supernatant IL-6 by the addition of anti-IL-6 antibody inhibited the neuronal differentiation and decreased cellular redox. Ionizing radiation and serum may act synergistically as neurotropic factors.
在血清存在的情况下，电离辐射可诱导PC12细胞分化为神经元样形态。这种效应在5戈瑞（Gy）时即可检测到，在10 - 20 Gy时达到最大值。在15 Gy剂量下，观察到核因子κB（NF-κB）的DNA结合活性增加以及白细胞介素6（IL-6）mRNA水平升高。通过添加抗IL-6抗体中和上清液中的IL-6可抑制神经元分化并降低细胞氧化还原状态。电离辐射和血清可能作为神经营养因子发挥协同作用。
