Abendroth C S, Dabbs D J
Department of Pathology, College of Medicine, Pennsylvania State University, Hershey, USA.
Acta Cytol. 1995 May-Jun;39(3):379-86.
Immunoperoxidase (IP) staining of fine needle aspiration specimens may provide useful adjunct information in cytodiagnosis. IP may be used on unstained direct smears or cell block material if available. In the absence of such material, IP is sometimes used on previously stained (Papanicolaou) smears. Few studies have examined the adequacy of such preparations, however. We compared IP staining results on unstained, alcohol-fixed or air-dried smears versus alcohol-fixed, previously Papanicolaou stained smears and versus alcohol-fixed, decolorized, Papanicolaou-stained smears, using antibodies to keratin (CAM 5.2, AE1/AE3 and K903), epithelial membrane antigen, monoclonal carcinoembryonic antigen, desmin, muscle-specific actin (HHF-35), vimentin, leukocyte common antigen, B and T cell markers L26 and UCHL-1, and prostate-specific antigen. Seventy-five sets of smears were made from various neoplastic or nonneoplastic tissues received as surgical specimens, and antibodies were selected for anticipated positive staining. The results were graded in a semiquantitative fashion. All the methods gave comparable results regarding expected positive staining, background staining and preservation of cellularity. We conclude that these methods of smear preparation are of equal utility in adjunctive immunocytochemical studies.
细针穿刺标本的免疫过氧化物酶（IP）染色可能在细胞诊断中提供有用的辅助信息。如果有未染色的直接涂片或细胞块材料，IP可用于其上。如果没有此类材料，IP有时可用于先前染色（巴氏染色）的涂片。然而，很少有研究检验过此类制片的充分性。我们比较了未染色、酒精固定或风干涂片、酒精固定且先前巴氏染色的涂片以及酒精固定、脱色且巴氏染色的涂片上的IP染色结果，使用了针对角蛋白（CAM 5.2、AE1/AE3和K903）、上皮膜抗原、单克隆癌胚抗原、结蛋白、肌肉特异性肌动蛋白（HHF-35）、波形蛋白、白细胞共同抗原、B和T细胞标志物L26和UCHL-1以及前列腺特异性抗原的抗体。从作为手术标本接收的各种肿瘤性或非肿瘤性组织制备了75组涂片，并选择了预期呈阳性染色的抗体。结果采用半定量方式分级。关于预期的阳性染色、背景染色和细胞性的保存，所有方法都给出了可比的结果。我们得出结论，这些涂片制备方法在辅助免疫细胞化学研究中具有同等效用。
