Yamaguchi K, Ogita K, Nakamura S, Nishizuka Y
Department of Biochemistry, Kobe University School of Medicine, Japan.
Biochem Biophys Res Commun. 1995 May 25;210(3):639-47. doi: 10.1006/bbrc.1995.1708.
Stimulation of CHO cells with phorbol ester caused transient activation of mitogen-activated protein kinase (MAP-kinases with 42 and 44 kDa). A similar treatment of CHO cells which overexpress a massive amount of the alpha- or delta-isoform of protein kinase C (PKC) resulted in the prolonged activation of MAP-kinase and eventually in the appearance of mostly dikaryotic, sometimes tetrakaryotic cells. The results suggest that the delta-isoform, as well as the alpha-isoform, has a potential to cause MAP kinase activation. Unusual activation of the PKC/MAP-kinase pathway, however, may lead to abnormal cytokinesis.
用佛波酯刺激CHO细胞会导致丝裂原活化蛋白激酶（42 kDa和44 kDa的MAP激酶）的瞬时激活。对过量表达大量蛋白激酶C（PKC）α或δ亚型的CHO细胞进行类似处理，会导致MAP激酶的持续激活，并最终出现大多为双核、有时为四核的细胞。结果表明，δ亚型以及α亚型都有可能导致MAP激酶激活。然而，PKC/MAP激酶途径的异常激活可能会导致异常的胞质分裂。
