Gill S, McManus A P, Crew A J, Benjamin H, Sheer D, Gusterson B A, Pinkerton C R, Patel K, Cooper C S, Shipley J M
Section of Molecular Carcinogenesis, Haddow Laboratories, Sutton, Surrey, United Kingdom.
Genes Chromosomes Cancer. 1995 Apr;12(4):307-10. doi: 10.1002/gcc.2870120412.
Southern blot analyses revealed a rearrangement of the EWS gene in a skeletal human myxoid chondrosarcoma. Interphase fluorescence in situ hybridization (FISH) studies, using cosmid clones F7 and G9 that flank the EWS locus on 22q12, confirmed the presence of this EWS gene abnormality. Cloning the rearranged EWS DNA fragment and mapping by FISH demonstrated that the EWS gene is joined to DNA sequences localised in 9q22-31. These findings are consistent with previous cytogenetic reports of a recurrent t(9;22)(q22-31;q11-12) in the myxoid variant of chondrosarcoma and reveal involvement of the EWS gene in a fourth type of human sarcoma.
Southern印迹分析显示，在一例人骨黏液样软骨肉瘤中EWS基因发生了重排。使用位于22q12上EWS基因座两侧的黏粒克隆F7和G9进行的间期荧光原位杂交（FISH）研究，证实了该EWS基因异常的存在。克隆重排的EWS DNA片段并通过FISH定位表明，EWS基因与定位于9q22 - 31的DNA序列相连。这些发现与先前关于软骨肉瘤黏液样变异型中复发性t(9;22)(q22 - 31;q11 - 12)的细胞遗传学报告一致，并揭示了EWS基因参与了第四种类型的人类肉瘤。
