Chandrashekar R, Curtis K C, Weil G J
Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
J Infect Dis. 1995 Jun;171(6):1586-92. doi: 10.1093/infdis/171.6.1586.
Onchocerca volvulus is a nematode that causes severe dermatitis and blindness in humans. Prior studies have shown that immune complexes in sera from onchocerciasis patients contain parasite antigens with M(r) of 23,000 and 65,000-70,000. Monoclonal antibody OV-1 binds to these antigens and to corresponding antigens in adult worm extracts and in vitro culture supernatants. OV-1 was used to immunoscreen an O. volvulus adult worm cDNA library. Clone OV1CF contains a 1632-bp open-reading frame that codes for a protein with a predicted M(r) of 63,000. The deduced protein sequence of OV1CF has 78% identity with Ascaris lumbricoides intermediate filament A and 40%-50% identity with several mammalian intermediate filaments. Antibodies raised to OV1CF bind to human keratin, and some sera from onchocerciasis patients contain antibodies to OV1CF and to keratin. Thus, immune complexes from onchocerciasis patients contain a parasite antigen that is immunologically cross-reactive with human intermediate filament proteins.
盘尾丝虫是一种可导致人类严重皮炎和失明的线虫。先前的研究表明，盘尾丝虫病患者血清中的免疫复合物含有分子量为23,000以及65,000 - 70,000的寄生虫抗原。单克隆抗体OV - 1可与这些抗原以及成虫提取物和体外培养上清液中的相应抗原结合。利用OV - 1对盘尾丝虫成虫cDNA文库进行免疫筛选。克隆OV1CF含有一个1632 bp的开放阅读框，编码一种预测分子量为63,000的蛋白质。OV1CF推导的蛋白质序列与蛔虫中间丝A有78%的同一性，与几种哺乳动物中间丝有40% - 50%的同一性。针对OV1CF产生的抗体可与人角蛋白结合，一些盘尾丝虫病患者的血清中含有针对OV1CF和角蛋白的抗体。因此，盘尾丝虫病患者的免疫复合物含有一种与人类中间丝蛋白具有免疫交叉反应性的寄生虫抗原。
