Sodhi A, Shrivastava A, Kumar R
School of Biotechnology, Banaras Hindu University, Varanasi, India.
Biochem Mol Biol Int. 1995 Mar;35(3):559-65.
The cellular and molecular interaction between monocyte/macrophage and tumor cells leading to macrophage activation is not clearly understood. Since protein tyrosine phosphorylation appears to be a major intracellular signalling event, we checked whether the tumor cells alter tyrosine phosphorylation of proteins in macrophages. We found that both L929 and Yac-1 tumor cells induced increased tyrosine phosphorylation of several polypeptides in peritoneal as well as P388D-1 and IC-21 macrophages. Macrophages co-cultured with tumor cells also showed increased fluorescence with anti-phosphotyrosine-FITC antibody. These observations suggest that increased tyrosine phosphorylation plays a role in tumor cell-induced activation of macrophages.
单核细胞/巨噬细胞与肿瘤细胞之间导致巨噬细胞活化的细胞和分子相互作用尚未完全明确。由于蛋白质酪氨酸磷酸化似乎是主要的细胞内信号事件，我们检测了肿瘤细胞是否会改变巨噬细胞中蛋白质的酪氨酸磷酸化。我们发现L929和Yac-1肿瘤细胞均可诱导腹膜巨噬细胞以及P388D-1和IC-21巨噬细胞中几种多肽的酪氨酸磷酸化增加。与肿瘤细胞共培养的巨噬细胞在用抗磷酸酪氨酸-FITC抗体检测时也显示出荧光增强。这些观察结果表明，酪氨酸磷酸化增加在肿瘤细胞诱导的巨噬细胞活化中发挥作用。
