Etienne E, Créminon C, Lamourette P, Grassi J, Pradelles P
CEA, Service de Pharmacologie et d'Immunologie, Département de Recherche en Imagerie, Pharmacologie et Physiologie, Gif sur Yvette, France.
Anal Biochem. 1995 Feb 10;225(1):34-8. doi: 10.1006/abio.1995.1104.
A new enzyme immunometric assay for L-thyroxine using uv irradiation as a cross-linking procedure is described. L-Thyroxine in plasma samples is immunocaptured by a monoclonal anti-L-thyroxine antibody coated on 96-well microtiter plates. After uv irradiation and methanol treatment, the covalently linked L-thyroxine is measured using the same monoclonal anti-L-thyroxine antibody labeled with acetylcholinesterase. A minimal detectable concentration of 4.8 nmol/liter was observed with a coefficient of variation less than 16% in the 20-320 nmol/liter. Specificity of the assay was very satisfying and a good correlation (r = 0.959) was noted for 33 human plasma samples between this assay and a commercial competitive radioimmunoassay.
描述了一种使用紫外线照射作为交联程序的新型L-甲状腺素酶免疫测定法。血浆样本中的L-甲状腺素通过包被在96孔微量滴定板上的抗L-甲状腺素单克隆抗体进行免疫捕获。经过紫外线照射和甲醇处理后，使用标记有乙酰胆碱酯酶的相同抗L-甲状腺素单克隆抗体来测量共价连接的L-甲状腺素。观察到最低检测浓度为4.8 nmol/升，在20 - 320 nmol/升范围内变异系数小于16%。该测定法的特异性非常令人满意，对于33份人类血浆样本，该测定法与商业竞争性放射免疫测定法之间具有良好的相关性（r = 0.959）。
