Keller R, Keist R, Joller P, Mülsch A
Department of Pathology, University of Zurich, Switzerland.
Biochem Biophys Res Commun. 1995 Jun 6;211(1):183-9. doi: 10.1006/bbrc.1995.1794.
Simultaneous incubation of primary rat bone-marrow-derived mononuclear phagocytes (BMMo) and tumor cells with gram-negative agents triggers within 24 h interferon gamma (IFN gamma)- and tumor necrosis factor (TNF alpha)-independent tumoricidal activity. On the other hand, BMMo that had been incubated for 24 h with gram-negative agents prior to re-exposure to the same agent had largely lost their ability to generate tumoricidal activity, although their ability to bind lipopolysaccharide (LPS) was not diminished. Parallel measurements of the kinetics of inducible nitric oxide synthase (iNOS), nitrite secretion, and tumoricidal activity triggered in primary BMMo by LPS revealed that these parameters take a coordinate course, reaching a peak within 24 h and then rapidly decaying. Down-regulation of expression of NOS protein and iNOS activity could be attributed neither to down-regulation of LPS receptors nor to L-arginine depletion.
将原代大鼠骨髓来源的单核吞噬细胞（BMMo）与肿瘤细胞同时用革兰氏阴性菌制剂孵育，可在24小时内引发不依赖干扰素γ（IFNγ）和肿瘤坏死因子（TNFα）的杀肿瘤活性。另一方面，在再次暴露于相同制剂之前已用革兰氏阴性菌制剂孵育24小时的BMMo，尽管其结合脂多糖（LPS）的能力未减弱，但其产生杀肿瘤活性的能力已大幅丧失。对LPS在原代BMMo中引发的诱导型一氧化氮合酶（iNOS）动力学、亚硝酸盐分泌和杀肿瘤活性进行的平行测量表明，这些参数呈现协同变化过程，在24小时内达到峰值，然后迅速衰减。NOS蛋白表达和iNOS活性的下调既不能归因于LPS受体的下调，也不能归因于L-精氨酸的耗竭。
