轴突运输蛋白聚糖的进一步特征研究。

Further characterization of axonally transported proteoglycans.

作者信息

Elam J S

机构信息

Department of Biological Science, Florida State University, Tallahassee 32306-3050, USA.

出版信息

Neurochem Res. 1995 Feb;20(2):187-93. doi: 10.1007/BF00970543.

DOI:10.1007/BF00970543

PMID:7540260

Abstract

We report further analysis of axonally transported proteoglycans in soluble and membranous subfractions of goldfish optic tectum. Distribution of transported 35SO4 radioactivity was 35.2% soluble, 63.4% Triton-NaCl extractable and 1.4% unextracted. Proteoglycans isolated on DEAE cellulose were treated with chondroitinase AC or nitrous acid and remaining heparan sulfate proteoglycans (HSPGs) and chondroitin sulfate proteoglycans (CSPGs) were sized on Sepharose CL-6B. Kav values and estimated molecular weights were: Soluble CSPG-0.36 (160 kDa), Triton-NaCl extracted CSPG-.031 (200 kDa), Soluble HSPG-0.37 (150 kDa), Triton-NaCl extracted HSPG-0.37 (150 kDa). For constituent CS and HS chains the Kav values and estimated molecular weights on CL-6B were: Soluble CS-0.55 (15 kDa), Triton-NaCl extracted CS-0.55 (15 kDa), Soluble HS-0.59 (13 kDa) and Triton-NaCl extracted HS-0.65 (9 kDa). CS was shown to be sulfated exclusively at carbon 4 for both soluble and Triton NaCl extracted fractions.

摘要

我们报告了对金鱼视顶盖可溶性和膜性亚组分中轴突运输的蛋白聚糖的进一步分析。运输的35SO4放射性分布为：35.2%可溶，63.4%可被Triton-NaCl提取，1.4%未被提取。在DEAE纤维素上分离的蛋白聚糖用软骨素酶AC或亚硝酸处理，剩余的硫酸乙酰肝素蛋白聚糖（HSPG）和硫酸软骨素蛋白聚糖（CSPG）在琼脂糖CL-6B上进行大小分级。Kav值和估计的分子量为：可溶性CSPG - 0.36（160 kDa），Triton-NaCl提取的CSPG - 0.31（200 kDa），可溶性HSPG - 0.37（150 kDa），Triton-NaCl提取的HSPG - 0.37（150 kDa）。对于组成性的CS和HS链，在CL-6B上的Kav值和估计的分子量为：可溶性CS - 0.55（15 kDa），Triton-NaCl提取的CS - 0.55（15 kDa），可溶性HS - 0.59（13 kDa），Triton-NaCl提取的HS - 0.65（9 kDa）。对于可溶性和Triton NaCl提取的组分，CS均显示仅在碳4位硫酸化。