Xu R K, Zhou Y Z, Huang M Y, Shan H M, Wang Y, Guo C H, Han X S
Department of Physiology, CAMS and PUMC, Beijing.
Sheng Li Xue Bao. 1995 Feb;47(1):65-72.
The role of brain-gut peptide galanin in the regulation of prolactin (PRL) and beta-endorphin (beta-EP) release from anterior pituitary lobe was studied in vivo in conscious male rats and in vitro with cultured anterior pituitary cells of the rat. Galanin (1 microgram or 3 micrograms/rat) injected into the third cerebral ventricle of rats produced highly significant, dose-related increases of PRL resting secretion, but did not alter resting secretion of beta-EP and restraint stress-induced release of PRL and beta-EP. However, galanin (0.05, 0.5 and 1.0 micrograms/5 x 10(5) cells.ml-1) induced highly significant, dose-related increase of beta-EP secretion from dispersed anterior pituitary cells, but failed to alter significantly PRL secretion from the cultured cells. These results indicate that central galanin has a stimulatory role in pituitary PRL resting secretion via the hypothalamus, whereas peripheral galanin stimulates beta-EP secretion only via direct action of this peptide in anterior pituitary cells.
在清醒雄性大鼠体内以及利用大鼠垂体前叶培养细胞进行体外实验，研究了脑肠肽甘丙肽在调节垂体前叶催乳素（PRL）和β-内啡肽（β-EP）释放中的作用。向大鼠第三脑室注射甘丙肽（1微克或3微克/只大鼠）可使PRL基础分泌量显著增加，且呈剂量相关性，但不改变β-EP基础分泌量以及束缚应激诱导的PRL和β-EP释放。然而，甘丙肽（0.05、0.5和1.0微克/5×10⁵细胞·ml⁻¹）可使分散的垂体前叶细胞β-EP分泌量显著增加，且呈剂量相关性，但对培养细胞的PRL分泌无显著影响。这些结果表明，中枢甘丙肽通过下丘脑对垂体PRL基础分泌具有刺激作用，而外周甘丙肽仅通过该肽对垂体前叶细胞的直接作用刺激β-EP分泌。
