Bellows S D, Hale S L, Simkhovich B Z, Kay G L, Kloner R A
Heart Institute, Hospital of the Good Samaritan, Los Angeles, CA 90017-2395, USA.
Cardiovasc Drugs Ther. 1995 Feb;9(1):117-23. doi: 10.1007/BF00877751.
There is controversy concerning the ability of antioxidant vitamins to reduce myocardial infarct size. We sought to determine whether a brief prophylactic treatment of vitamin C or vitamin C plus Trolox (a water-soluble form of vitamin E) could reduce myocardial infarct size in an experimental model. We used an anesthetized open-chest rabbit model in which a branch of the circumflex coronary artery was ligated for 30 minutes followed by 4 hours of reperfusion. Experiments were performed in a randomized and blinded fashion. An IV injection of normal saline pH balanced to 7.4 (control group n = 15), vitamin C (150 mg/kg, n = 14), or vitamin C plus Trolox (150 mg/kg plus 100 mg/kg, respectively, n = 15) was administered prior to coronary occlusion. Collateral blood flow during coronary occlusion was measured by radioactive microspheres, myocardial risk zone (AR) was assessed by blue dye injection, and myocardial infarct size (AN) was assessed by triphenyltetrazolium chloride staining. All rabbits received comparable ischemic insult: Collateral blood flow and AR were similar among all three groups. Infarct size, measured as a percent of AR, did not differ significantly among the controls (21%), vitamin C (29%), or the vitamin C plus Trolox (18%) groups. Therefore, in this ischemia/reperfusion model, antioxidant vitamins did not alter myocardial infarct size.
关于抗氧化维生素能否减小心肌梗死面积存在争议。我们试图确定，短期预防性给予维生素C或维生素C加生育酚（维生素E的一种水溶性形式）是否能在实验模型中减小心肌梗死面积。我们使用了一种麻醉开胸兔模型，在此模型中，左旋冠状动脉的一个分支被结扎30分钟，随后再灌注4小时。实验以随机、盲法进行。在冠状动脉闭塞前静脉注射pH值平衡至7.4的生理盐水（对照组n = 15）、维生素C（150 mg/kg，n = 14）或维生素C加生育酚（分别为150 mg/kg加100 mg/kg，n = 15）。冠状动脉闭塞期间的侧支血流通过放射性微球测量，心肌危险区（AR）通过注射蓝色染料评估，心肌梗死面积（AN）通过氯化三苯基四氮唑染色评估。所有兔子均受到相当的缺血损伤：三组之间的侧支血流和AR相似。以AR的百分比衡量的梗死面积在对照组（21%）、维生素C组（29%）或维生素C加生育酚组（18%）之间无显著差异。因此，在这个缺血/再灌注模型中，抗氧化维生素并未改变心肌梗死面积。
