Benzing W C, Mufson E J
Department of Neurological Sciences, Rush Presbyterian St. Luke's Medical Center, Chicago, Illinois 60612, USA.
Exp Neurol. 1995 Apr;132(2):162-71. doi: 10.1016/0014-4886(95)90021-7.
The present immunohistochemical study determined the relationship between ApoE and the expression of the cytoskeletal protein tau (Tau2) and paired helical filaments (PHF), within the magnocellular neurons of the nucleus basalis of Meynert and layer II stellate neurons of the entorhinal cortex in Alzheimer's disease (AD). Although nearly all ApoE immunoreactive perikarya within these two brain regions were PHF immunoreactive, not all PHF and Tau2 containing neurons stained for ApoE in AD. Moreover, more Tau2-immunostained neurons, as compared to PHF, were ApoE immunonegative. This was particularly evident in a population of control subjects which exhibited AD-like pathology intermediate between the AD and normal aged individuals. Thus, neurons within the nucleus basalis of Meynert and entorhinal cortex layer II stellate exhibit evidence of cytoskeletal pathology prior to displaying ApoE. These observations suggest that (1) ApoE plays a secondary role in NFT formation or (2) this protein is accumulated within these neurons in response to reparative process(es) induced by NFT-associated neuronal damage.
本免疫组织化学研究确定了阿尔茨海默病（AD）中，载脂蛋白E（ApoE）与梅纳特基底核大细胞神经元及内嗅皮层II层星状神经元中细胞骨架蛋白tau（Tau2）和双螺旋丝（PHF）表达之间的关系。尽管这两个脑区中几乎所有ApoE免疫反应阳性的核周体均为PHF免疫反应阳性，但在AD中，并非所有含PHF和Tau2的神经元都对ApoE染色。此外，与PHF相比，更多Tau2免疫染色的神经元ApoE免疫阴性。这在一群表现出介于AD和正常老年人之间的AD样病理的对照受试者中尤为明显。因此，梅纳特基底核和内嗅皮层II层星状神经元在显示ApoE之前就表现出细胞骨架病理的证据。这些观察结果表明：（1）ApoE在神经原纤维缠结（NFT）形成中起次要作用；或（2）该蛋白在这些神经元中积累是对NFT相关神经元损伤诱导的修复过程的反应。
