Lee M E, Westrum L E, Hendricks K R, Leonard J R, Kott J N
Department of Neurological Surgery, University of Washington, Seattle 98195, USA.
Exp Neurol. 1995 Apr;132(2):284-90. doi: 10.1016/0014-4886(95)90034-9.
We are using wheat germ agglutinin-conjugated horseradish peroxidase (WHRP) to study reconnectivity in the transplanted (TX) olfactory bulb (OB) in Sprague-Dawley rats. Tritium-labeled OBs from fetal rat donors of Embryonic Days 14-15 were immediately grafted into neonatal rats in the site from which the host OB had been removed. Following survival times of 7 weeks and longer, WHRP solution was injected into the TX OB, and subjects were perfused after 24 h. The WHRP transport is seen in fibers from the TX OB into layer I of the host olfactory peduncle (OP) and olfactory cortex (OC) and in cell bodies in layers II and III of the OP and OC, the lateral hypothalamus, and the contralateral anterior olfactory nucleus (AON). These findings reaffirm that the axons from a TX OB make connections with some appropriate areas of the host brain and also indicate that axons from cells in the target areas of the host brain, including contralateral AON, reinnervate the TX OB.
我们正在使用小麦胚芽凝集素结合辣根过氧化物酶（WHRP）来研究Sprague-Dawley大鼠移植（TX）嗅球（OB）中的重新连接性。来自胚胎第14 - 15天胎鼠供体的氚标记嗅球立即被移植到新生大鼠体内宿主嗅球已被移除的部位。在存活7周及更长时间后，将WHRP溶液注入TX嗅球，24小时后对实验对象进行灌注。在从TX嗅球延伸至宿主嗅柄（OP）和嗅皮质（OC）第I层的纤维中，以及在OP和OC的第II和III层、外侧下丘脑和对侧前嗅核（AON）的细胞体中可观察到WHRP的运输。这些发现再次证实来自TX嗅球的轴突与宿主脑的一些适当区域建立了连接，并且还表明来自宿主脑靶区域（包括对侧AON）细胞的轴突重新支配了TX嗅球。
