Ewing C, Topham D J, Doherty P C
Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Virology. 1995 Jun 20;210(1):179-85. doi: 10.1006/viro.1995.1329.
The relative prevalence of Sendai virus-specific CD4+ T helper precursors (Thp) has been assessed by limiting dilution analysis (LDA). Within 10 days after intranasal inoculation of C57BL/6 mice, Thp prevalence increased from < 1 in 10(5) to approximately 1 in 200 in CD4+ T-cell-enriched spleen and mediastinal lymph node populations. These frequencies remained elevated relative to naive animals, with the majority of the Thp recovered at 2-3 months after infection being found in the spleen. The "memory" Thp express an "activated" L-selectin-low, CD44-high, alpha 4-integrin-high phenotype, comparable to that described previously for CD8+ cytotoxic T lymphocyte precursors (CTLp) specific for Sendai virus. Background effects for the IL-2-based Thp analysis are, however, less predictable than those found previously for the 51Cr release CTLp LDA, as the extent of non-virus-specific lymphokine production varies. However, provided the analysis is appropriately controlled, the approach does allow useful comparisons between phenotypically different subsets of antigen-specific CD4+ T cells.
通过有限稀释分析（LDA）评估了仙台病毒特异性CD4 + T辅助前体细胞（Thp）的相对患病率。在对C57BL / 6小鼠进行鼻内接种后的10天内，在富含CD4 + T细胞的脾脏和纵隔淋巴结群体中，Thp患病率从每10⁵个细胞中少于1个增加到约每200个细胞中1个。相对于未感染动物，这些频率仍然升高，感染后2 - 3个月恢复的Thp大部分存在于脾脏中。“记忆”Thp表达“活化”的低L-选择素、高CD44、高α4整合素表型，这与先前描述的仙台病毒特异性CD8 +细胞毒性T淋巴细胞前体细胞（CTLp）的表型相当。然而，基于IL-2的Thp分析的背景效应比先前用于⁵¹Cr释放CTLp LDA的背景效应更难预测，因为非病毒特异性淋巴因子产生的程度各不相同。但是，只要分析得到适当控制，该方法确实可以在抗原特异性CD4 + T细胞的表型不同亚群之间进行有用的比较。
