通过聚合酶链反应体外扩增Y染色体基因监测大鼠原位肝移植后的植入情况。

Monitoring for engraftment following rat orthotopic liver transplantation by in vitro amplification of Y-chromosome gene using polymerase chain reaction.

作者信息

Tashiro H, Fukuda Y, Hoshino S, Furukawa M, Shintaku S, Dohi K

机构信息

Second Department of Surgery and Bacteriology, Hiroshima University School of Medicine.

出版信息

Cell Transplant. 1995;4 Suppl 1:S61-3. doi: 10.1016/0963-6897(94)00060-w.

DOI:10.1016/0963-6897(94)00060-w

PMID:7540919

Abstract

The polymerase chain reaction (PCR) using primers specific for the rat Y-chromosome gene made it possible to distinguish a very small number of male rat cells from a large excess of female rat cells. In nonimmunosuppressed LEW recipients of ACI liver allografts, the donor cells in the bloodstream disappeared rapidly by day 3, earlier than the biochemical changes indicative of liver dysfunction. In immunosuppressed LEW recipients of ACI liver allografts, the donor cells were detected for a longer time. Moreover, in LEW recipients surviving for long period, the PCR revealed mixed-microchimerism. Our results indicated that this Y chromosomal gene-specific PCR method is useful for assessing engraftment following rat liver transplantation.

摘要

使用针对大鼠Y染色体基因的特异性引物进行聚合酶链反应（PCR），使得从大量雌性大鼠细胞中区分出极少数雄性大鼠细胞成为可能。在未免疫抑制的接受ACI肝同种异体移植的LEW受体中，血流中的供体细胞在第3天迅速消失，早于指示肝功能障碍的生化变化。在免疫抑制的接受ACI肝同种异体移植的LEW受体中，供体细胞被检测到的时间更长。此外，在长期存活的LEW受体中，PCR显示出混合微嵌合体现象。我们的结果表明，这种Y染色体基因特异性PCR方法可用于评估大鼠肝移植后的植入情况。