Laban O, Dimitrijević M, von Hoersten S, Marković B M, Janković B D
Immunology Research Center, Belgrade, Yugoslavia.
Brain Res. 1995 Apr 3;676(1):133-40. doi: 10.1016/0006-8993(95)00106-z.
The present study investigated the effect of daily handling and gentling between postnatal days 1 and 28 on experimental allergic encephalomyelitis (EAE) in 8-week old DA rats. Handling consisted of removing pups from the mother, and placing them in the novel cage for 15 min. The gentling procedure included handling accompanied by 3 min of dorsal tactile stimulation before returning the pups to the nest cage. Adult rats of both sexes handled in infancy showed increased susceptibility to EAE, as revealed by higher incidence of the disease, and more severe clinical signs. Anti-myelin basic protein (MBP) autoantibodies were increased in handled males, and decreased in handled females, compared to controls. Gentling induced aggravation of clinical signs and histopathological lesions of EAE in males, while in gentled females suppression was observed. These results indicated that both neonatal handling and gentling aggravated EAE induced in adult male rats. In female rats handling exacerbated, and gentling suppressed clinical EAE. The overall effect of neonatal manipulations was more pronounced in males. Furthermore, in mothers separated from their offspring due to handling and gentling, and immunized for EAE at day 28 postpartum, earlier appearance of clinical signs, and increased frequency of relapses compared to control dams was recorded.
本研究调查了出生后第1天至第28天每天进行的抚摸和轻柔操作对8周龄DA大鼠实验性变态反应性脑脊髓炎（EAE）的影响。抚摸操作包括将幼崽从母鼠身边取出，放入新笼子中15分钟。轻柔操作包括在将幼崽放回巢笼之前，在抚摸的同时进行3分钟的背部触觉刺激。与对照组相比，幼年时接受抚摸操作的成年雌雄大鼠对EAE的易感性增加，表现为疾病发病率更高，临床症状更严重。与对照组相比，接受抚摸操作的雄性大鼠抗髓鞘碱性蛋白（MBP）自身抗体增加，而雌性大鼠则减少。轻柔操作导致雄性大鼠EAE的临床症状和组织病理学损伤加重，而在接受轻柔操作的雌性大鼠中则观察到抑制作用。这些结果表明，新生期的抚摸和轻柔操作均加重了成年雄性大鼠诱发的EAE。在雌性大鼠中，抚摸操作加剧了EAE，而轻柔操作则抑制了临床EAE。新生期操作的总体影响在雄性大鼠中更为明显。此外，由于抚摸和轻柔操作而与后代分离、并在产后第28天接受EAE免疫的母鼠，与对照母鼠相比，临床症状出现更早，复发频率增加。
