Saetrum Opgaard O, Gulbenkian S, Bergdahl A, Barroso C P, Andrade N C, Polak J M, Queiroz e Melo J Q, Edvinsson L
Department of Internal Medicine, University Hospital of Lund, Sweden.
Cardiovasc Res. 1995 Apr;29(4):463-8. doi: 10.1016/0008-6363(96)88520-8.
The aim was to investigate the innervation and vasomotor responses to classical and putative transmitters of the coronary venous bed.
The innervation of human epicardial coronary veins was investigated using acetylcholinesterase histochemistry and immunofluorescence staining, together with antisera against the general neuronal marker protein gene product 9.5 (PGP 9.5), the catecholamine synthesising enzyme tyrosine hydroxylase, and neuropeptides [neuropeptide Y, vasoactive intestinal peptide (VIP), substance P, and calcitonin gene related peptide (CGRP)]. The vasomotor responses to noradrenaline, acetylcholine, neuropeptide Y, substance P, human alpha calcitonin gene related peptide (alpha CGRP), and VIP were tested on isolated circular human epicardial coronary vein segments.
A network of nerve fibres was shown in the major epicardial coronary veins by means of an antiserum to PGP 9.5. The majority of the perivascular nerve fibres possessed neuropeptide Y and tyrosine hydroxylase immunoreactivity. Only a few nerve fibres displayed substance P, CGRP, and VIP immunoreactivity and acetylcholinesterase activity. Noradrenaline and acetylcholine induced powerful contractions of all the tested segments, whereas no contraction was induced by neuropeptide Y, alpha CGRP, substance P, or VIP. All segments precontracted with U46619 responded with potent relaxation to alpha CGRP, substance P, and VIP, whereas noradrenaline and acetylcholine only in low concentrations induced weak relaxation of a few of the segments. No relaxation was induced by neuropeptide Y.
This is the first study to demonstrate comprehensively the perivascular innervation of human coronary veins and corresponding vasomotor effects, suggesting a role in regulation of the coronary venous circulation.
旨在研究冠状静脉床对经典递质和假定递质的神经支配及血管舒缩反应。
采用乙酰胆碱酯酶组织化学和免疫荧光染色，结合针对一般神经元标志物蛋白基因产物9.5（PGP 9.5）、儿茶酚胺合成酶酪氨酸羟化酶以及神经肽[神经肽Y、血管活性肠肽（VIP）、P物质和降钙素基因相关肽（CGRP）]的抗血清，研究人心外膜冠状静脉的神经支配。在离体的人环形心外膜冠状静脉段上测试去甲肾上腺素、乙酰胆碱、神经肽Y、P物质、人α降钙素基因相关肽（αCGRP）和VIP的血管舒缩反应。
通过抗PGP 9.5血清显示，主要的心外膜冠状静脉存在神经纤维网络。大多数血管周围神经纤维具有神经肽Y和酪氨酸羟化酶免疫反应性。仅有少数神经纤维显示P物质、CGRP和VIP免疫反应性以及乙酰胆碱酯酶活性。去甲肾上腺素和乙酰胆碱可引起所有测试段的强烈收缩，而神经肽Y、αCGRP、P物质或VIP未引起收缩。所有预先用U46619预收缩的段对αCGRP、P物质和VIP均有强力舒张反应，而去甲肾上腺素和乙酰胆碱仅在低浓度时可引起少数段的微弱舒张。神经肽Y未引起舒张。
这是首次全面证明人冠状静脉血管周围神经支配及相应血管舒缩效应的研究，提示其在冠状静脉循环调节中发挥作用。
