Nebe B, Rychly J, Knopp A, Bohn W
Department of Internal Medicine, University of Rostock, Germany.
Exp Cell Res. 1995 Jun;218(2):479-84. doi: 10.1006/excr.1995.1181.
Mechanical stress influences growth, differentiation, and gene expression in a variety of cell types. It is believed that via extracellular matrix the mechanical stimulus is transmitted to integrin receptors which thus play a key role in transducing signals into the cell interior. Here we demonstrate that incubation of suspended hepatocytes with specific antibodies to beta 1-integrin subunits followed by a short-term mechanical stimulation is sufficient to induce a rise in intracellular Ca2+. The results indicate that mechanical loading of individual integrin subunits activates Ca(2+)-specific signal pathways.
机械应力会影响多种细胞类型的生长、分化和基因表达。据信，机械刺激通过细胞外基质传递至整合素受体，因此整合素受体在将信号转导至细胞内部过程中发挥关键作用。在此，我们证明，用针对β1整合素亚基的特异性抗体孵育悬浮的肝细胞，随后进行短期机械刺激，足以诱导细胞内Ca2+浓度升高。结果表明，单个整合素亚基的机械负荷激活了Ca(2+)特异性信号通路。
