Manna S K, Samanta A K
Division of Immunobiology, Indian Institute of Chemical Biology, Calcutta.
FEBS Lett. 1995 Jun 26;367(2):117-21. doi: 10.1016/0014-5793(95)00525-e.
Interleukin-8 (IL-8) is implicated in the pathogenesis of a large number of neutrophil-driven inflammatory diseases. Although the cytokine activates neutrophils through a receptor, no information is available regarding the regulation of IL-8 receptor (IL-8R) expression. The present study shows that, compared to control, the bacterial products--formylpeptide and LPS (serum-activated) upregulate IL-8 receptor by 54% and 115%, respectively, the former by degranulation of the secretory vesicle and the latter by de novo protein synthesis. The newly expressed IL-8R could be demonstrated with anti-IL-8R-antibody and by autoradiogram of the receptor crosslinked with [125I]IL-8. The study may be useful for understanding the potential role of IL-8 during neutrophil mediated inflammatory response.
白细胞介素-8（IL-8）与多种由中性粒细胞驱动的炎症性疾病的发病机制有关。尽管这种细胞因子通过受体激活中性粒细胞，但关于白细胞介素-8受体（IL-8R）表达的调控尚无相关信息。本研究表明，与对照组相比，细菌产物——甲酰肽和脂多糖（血清激活型）分别使IL-8受体上调54%和115%，前者通过分泌囊泡脱颗粒，后者通过从头合成蛋白质。新表达的IL-8R可用抗IL-8R抗体以及与[125I]IL-8交联的受体放射自显影片来证实。该研究可能有助于理解IL-8在中性粒细胞介导的炎症反应中的潜在作用。
