Last R D, Jardine J E, Smit M M, van der Lugt J J
Allerton Regional Veterinary Laboratory, Pietermaritzburg, South Africa.
Onderstepoort J Vet Res. 1994 Jun;61(2):183-7.
Two immunoperoxidase techniques, viz. avidin-biotin complex (ABC) and peroxidase-anti-peroxidase (PAP) procedures, were applied to paraffin-wax-embedded brain-tissue sections, from brains which had been fixed in 10% formalin, to demonstrate the presence of rabies-virus antigen by light microscopy. These techniques positively identified both "viverrid" and "canid" rabies-virus antigen in tissues sections of species commonly infected with rabies virus in southern Africa, viz. the domestic dog (Canis familiaris), yellow mongoose (Cynictus penicillata), black-backed jackal (Canis mesomelas), bat-eared fox (Otocyon megalotus), cattle (Bos taurus), sheep (Ovis aries) and humans. With both of these techniques rabies-virus antigen stained as sharply demarcated, brown precipitates within the cytoplasm of neurons. The virtual absence of background staining enabled identification of fine granules of viral antigen, often referred to as "virus dust", within axons, dendrites and cytoplasm of the nerve cell body. Staining with the ABC procedure clearer, more deeply-coloured precipitates than the PAP method.
两种免疫过氧化物酶技术，即抗生物素蛋白-生物素复合物（ABC）法和过氧化物酶-抗过氧化物酶（PAP）法，应用于用10%福尔马林固定的大脑石蜡包埋组织切片，通过光学显微镜检测狂犬病病毒抗原的存在。这些技术在南非通常感染狂犬病病毒的物种的组织切片中，成功鉴定出“灵猫科”和“犬科”狂犬病病毒抗原，这些物种包括家犬（犬属家犬）、黄獴（食蟹獴）、黑背胡狼（犬属黑背胡狼）、蝙蝠耳狐（苍狐）、牛（牛属黄牛）、绵羊（绵羊属绵羊）和人类。使用这两种技术时，狂犬病病毒抗原均染成界限清晰的棕色沉淀物，位于神经元细胞质内。几乎没有背景染色，从而能够在轴突、树突和神经细胞体的细胞质内识别出通常被称为“病毒尘”的病毒抗原细颗粒。与PAP法相比，ABC法染色产生的沉淀物更清晰、颜色更深。
