Haas N, Hamann K, Grabbe J, Algermissen B, Czarnetzki B M
Department of Dermatology, UKRV, Freie Universität, Berlin, Germany.
Arch Dermatol Res. 1995;287(3-4):242-8. doi: 10.1007/BF01105073.
In order to identify possible cellular abnormalities in human mastocytosis, sections from 13 urticaria pigmentosa lesions and 5 mastocytomas were compared with 5 normal skin specimens using histochemical, enzyme histochemical and immunohistochemical techniques. All toluidine blue-positive mast cells also reacted with Fc epsilon RI and c-kit antibodies, almost all stained for tryptase, many for chymase and the myeloid workshop mast cell antibodies, few for Fc epsilon RII and none for the proliferation marker Ki-67. Urticaria pigmentosa lesions contained fewer epidermal Langerhans cells and a lower percentage of avidin-positive mast cells than mastocytomas and normal skin. Mastocytomas exhibited generally weaker staining for mast cell markers and mostly lacked Fc epsilon RI-bound IgE on mast cells and Langerhans cells, although the receptor was able to bind IgE in tissue sections. Most of the mast cell antibodies also reacted with other cell types. Only toluidine blue, avidin, tryptase and chymase stains were mast cell specific. Mast cells in mastocytosis thus differed only to a minor degree from normal mast cells, although distinct pathomechanisms may play a role in urticaria pigmentosa and mastocytosis.
为了确定人类肥大细胞增多症中可能存在的细胞异常，运用组织化学、酶组织化学和免疫组织化学技术，将13个色素性荨麻疹皮损和5个肥大细胞瘤的切片与5个正常皮肤标本进行了比较。所有甲苯胺蓝阳性的肥大细胞也与FcεRI和c-kit抗体发生反应，几乎所有细胞对类胰蛋白酶染色阳性，许多细胞对糜蛋白酶和髓系车间肥大细胞抗体染色阳性，少数细胞对FcεRII染色阳性，而增殖标志物Ki-67染色均为阴性。与肥大细胞瘤和正常皮肤相比，色素性荨麻疹皮损中的表皮朗格汉斯细胞较少，抗生物素蛋白阳性肥大细胞的比例较低。肥大细胞瘤对肥大细胞标志物的染色通常较弱，肥大细胞和朗格汉斯细胞上大多缺乏与FcεRI结合的IgE，尽管该受体在组织切片中能够结合IgE。大多数肥大细胞抗体也与其他细胞类型发生反应。只有甲苯胺蓝、抗生物素蛋白、类胰蛋白酶和糜蛋白酶染色是肥大细胞特异性的。因此，肥大细胞增多症中的肥大细胞与正常肥大细胞仅有轻微差异，尽管不同的发病机制可能在色素性荨麻疹和肥大细胞增多症中起作用。
