Tittawella I
Department of Cell- & Molecular Biology, University of Umeå, Sweden.
Biochem Biophys Res Commun. 1995 Jun 26;211(3):892-900. doi: 10.1006/bbrc.1995.1896.
Riboprotein particles containing the ribosomal RNA-like 9S and 12S RNAs in the trypanosome mitochondrion have never been isolated. Using the formaldehyde cross-linking procedure I show here that one or more of three proteins, q2 (16.5 kDa), r (15 kDa) and s (13 kDa), are closely associated in vivo with the 9S and 12S RNAs of the trypanosomatid parasite, Crithidia fasciculata. These proteins were also found to be associated with the parasite's cytosolic ribosomal RNA and to have strong immunological cross reactivity with riboprotein S11 of the bacterium Escherichia coli. These data provide the first evidence for the association of riboprotein-like proteins with the 9S and 12S mitochondrial RNA in a trypanosome, possibly as components of a mitochondrial ribosome.
在锥虫线粒体中，含有核糖体RNA样9S和12S RNA的核糖核蛋白颗粒从未被分离出来。我在此使用甲醛交联程序表明，三种蛋白质q2（16.5 kDa）、r（15 kDa）和s（13 kDa）中的一种或多种在体内与锥虫寄生虫克氏锥虫的9S和12S RNA紧密相关。这些蛋白质也被发现与该寄生虫的胞质核糖体RNA相关，并且与大肠杆菌的核糖核蛋白S11具有强烈的免疫交叉反应性。这些数据首次证明了核糖蛋白样蛋白质与锥虫线粒体9S和12S RNA的关联，可能作为线粒体核糖体的组成部分。
