Maqbool A, Batten T F, McWilliam P N
Institute for Cardiovascular Research, Research School of Medicine, University of Leeds, UK.
J Chem Neuroanat. 1995 Mar;8(3):191-206. doi: 10.1016/0891-0618(94)00045-u.
The distribution of nitric oxide producing neurones in the medulla oblongata of the cat was investigated using nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry, and nitric oxide synthase (NOS) immunohistochemistry. The pattern of staining obtained with both methods was found to be similar. Strongly diaphorase and NOS reactive neurones were present in the paramedian and lateral tegmental fields, including the regions occupied by the A1/C1 catecholamine cell groups, the nucleus ambiguus and lateral reticular nucleus, and in a number of sensory nuclei including the nucleus of the tractus solitarius and the dorsal column nuclei. The extent of co-localization of NADPH-diaphorase with a number of neuropeptides and neurotransmitters was investigated by combining NADPH-diaphorase histochemistry with immunocytochemistry for neuropeptide Y, somatostatin, glutamate, cholecystokinin and tyrosine hydroxylase. NADPH-diaphorase reaction product was observed in neurones immunoreactive for glutamate and somatostatin. These double-labelled cells were found in the paramedian region, lateral reticular field, the nucleus prepositus hypoglossi and in the rostral nucleus of the tractus solitarius. In the rostral ventrolateral medulla NADPH-diaphorase/somatostatin immunoreactive cells were found in the paragigantocellular nucleus. NADPH-diaphorase/glutamate immunoreactive cells overlapped the nucleus ambiguus, the lateral reticular nucleus and the A1/C1 catecholaminergic cell groups. In addition, a few NADPH-diaphorase/glutamate immunoreactive cells were found in the paraolivary area and gigantocellular tegmental field, in the external cuneate and infratrigeminal nuclei. The functional implications of the co-localization of nitric oxide with these neurotransmitters in areas of the medulla concerned with cardiovascular regulation is discussed.
利用烟酰胺腺嘌呤二核苷酸磷酸（NADPH）黄递酶组织化学和一氧化氮合酶（NOS）免疫组织化学方法，研究了猫延髓中产生一氧化氮的神经元的分布。发现两种方法获得的染色模式相似。在中缝旁和外侧被盖区存在强黄递酶和NOS反应性神经元，包括A1/C1儿茶酚胺细胞群、疑核和外侧网状核所占据的区域，以及一些感觉核，包括孤束核和薄束核。通过将NADPH黄递酶组织化学与神经肽Y、生长抑素、谷氨酸、胆囊收缩素和酪氨酸羟化酶的免疫细胞化学相结合，研究了NADPH黄递酶与多种神经肽和神经递质的共定位程度。在对谷氨酸和生长抑素免疫反应的神经元中观察到NADPH黄递酶反应产物。这些双标记细胞见于中缝旁区域、外侧网状区、舌下前置核和孤束核吻侧核。在延髓吻侧腹外侧，在巨细胞旁核中发现了NADPH黄递酶/生长抑素免疫反应性细胞。NADPH黄递酶/谷氨酸免疫反应性细胞与疑核、外侧网状核和A1/C1儿茶酚胺能细胞群重叠。此外，在橄榄旁区和巨细胞被盖区、楔外核和三叉神经下核中发现了一些NADPH黄递酶/谷氨酸免疫反应性细胞。讨论了一氧化氮与这些神经递质在延髓心血管调节相关区域共定位的功能意义。
