Yamamoto K, Tsuji T, Osawa T
Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Mol Biotechnol. 1995 Feb;3(1):25-36. doi: 10.1007/BF02821332.
Lectins are proteins that specifically bind to a particular carbohydrate structure. Affinity chromatography with immobilized lectins is a quite effective technique not only for the fractionation of glycoproteins or oligosaccharides but also their structural assessment. In this article, we focus on the separation of glycopeptides and oligosaccharides derived from glycoproteins by affinity chromatography on immobilized lectin columns.
凝集素是一类能特异性结合特定碳水化合物结构的蛋白质。固定化凝集素亲和色谱法不仅是一种用于糖蛋白或寡糖分级分离的有效技术，也是用于其结构评估的有效技术。在本文中，我们重点关注通过固定化凝集素柱亲和色谱法分离糖蛋白衍生的糖肽和寡糖。
