Lifson B, Pizzolo J G, Elhosseiny A A, Choudhury M S, Melamed M R
Department of Urology, New York Medical College, Valhalla 10595, USA.
Anal Quant Cytol Histol. 1995 Apr;17(2):93-9.
Cancer cell nuclei extracted from 15 paraffin-embedded radical prostatectomy specimens were examined with fluorescent in situ hybridization (FISH) and flow cytometry (FCM) for DNA ploidy. Repetitive chromosome-specific alpha-satellite DNA probes to centromeres 7 and 10 were used in the FISH assay. Results from FISH and FCM were compared and viewed in relation to clinical experience. Of the 15 tumors examined, 9 were hyperdiploid for at least one chromosome by FISH assay. Seven were aneuploid by FCM, showing a good correlation between the two methods (P = .006). Using either evidence of clinical progression or a postoperative prostate-specific antigen (PSA) level > 0.5 ng/mL by Hybritech assay to indicate the risk of clinical progression, 56% of FISH hyperdiploid tumors had a risk of progression of carcinoma versus 17% of FISH diploid tumors. A preoperative PSA level > 30 ng/mL had a marginal correlation with risk of progression (P = .05). We demonstrate that FISH is a useful tool for chromosomal analysis in paraffin-embedded tumor specimens.
从15例石蜡包埋的前列腺癌根治术标本中提取癌细胞核，采用荧光原位杂交（FISH）和流式细胞术（FCM）检测DNA倍体。FISH检测中使用了针对7号和10号染色体着丝粒的重复性染色体特异性α卫星DNA探针。将FISH和FCM的结果与临床经验进行比较和分析。在检测的15个肿瘤中，通过FISH检测，9个肿瘤至少有一条染色体为超二倍体。7个肿瘤通过FCM检测为非整倍体，两种方法之间显示出良好的相关性（P = 0.006）。采用临床进展证据或Hybritech检测术后前列腺特异性抗原（PSA）水平>0.5 ng/mL来表明临床进展风险，FISH超二倍体肿瘤中有56%存在癌进展风险，而FISH二倍体肿瘤中这一比例为17%。术前PSA水平>30 ng/mL与进展风险有边缘相关性（P = 0.05）。我们证明FISH是石蜡包埋肿瘤标本染色体分析的有用工具。
