McAlonan G M, Dawson G R, Wilkinson L O, Robbins T W, Everitt B J
Department of Experimental Psychology, University of Cambridge, UK.
Eur J Neurosci. 1995 May 1;7(5):1034-49. doi: 10.1111/j.1460-9568.1995.tb01091.x.
These experiments investigated in the rat the impact on spatial delayed non-matching to sample and on acquisition of the Morris water maze of (i) AMPA-induced lesions of the medial septal nucleus, which produced a marked reduction of hippocampal choline acetyltransferase activity and acetylcholine levels (measured using in vivo dialysis) together with lesser reductions in cholinergic markers in the cingulate cortex and (ii) similar AMPA-induced lesions of the vertical limb nucleus of the diagonal band of Broca (vDB), which produced more marked reductions in cholinergic markers in the cingulate cortex than in the hippocampus. Medial septal lesions produced a delay-dependent deficit in spatial working memory, while lesions of the vDB resulted in a delay-independent performance deficit. In addition, rats with vDB lesions adopted biased response strategies during the imposition of long delays. Neither lesion significantly affected the acquisition of a spatial reference memory task, the Morris water maze. The results are discussed in terms of cholinergic- and GABAergic-dependent functions of the hippocampal formation and cingulate cortex in spatial short-term and reference memory.
（i）AMPA诱导的内侧隔核损伤，这导致海马胆碱乙酰转移酶活性和乙酰胆碱水平（通过体内透析测量）显著降低，同时扣带回皮质中胆碱能标志物的降低幅度较小；（ii）类似的AMPA诱导的布罗卡斜角带垂直支核（vDB）损伤，这导致扣带回皮质中胆碱能标志物的降低幅度比海马更显著。内侧隔核损伤导致空间工作记忆中与延迟相关的缺陷，而vDB损伤导致与延迟无关的行为表现缺陷。此外，vDB损伤的大鼠在施加长时间延迟期间采用了有偏差的反应策略。两种损伤均未显著影响空间参考记忆任务——莫里斯水迷宫的学习。从海马结构和扣带回皮质在空间短期记忆和参考记忆中胆碱能和GABA能依赖性功能的角度对结果进行了讨论。
