通过扩增编码rRNA的基因检测火鸡病原体火鸡支原体和衣阿华支原体。

Detection of the turkey pathogens Mycoplasma meleagridis and M. iowae by amplification of genes coding for rRNA.

作者信息

Boyle J S, Good R T, Morrow C J

机构信息

Victorian Institute of Animal Science, Department of Agriculture, Attwood, Australia.

出版信息

J Clin Microbiol. 1995 May;33(5):1335-8. doi: 10.1128/jcm.33.5.1335-1338.1995.

DOI:10.1128/jcm.33.5.1335-1338.1995

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228157/

Abstract

PCR-based diagnostic tests using oligonucleotides specific to 16S rRNA were designed for the specific detection of the turkey pathogens Mycoplasma meleagridis and M. iowae. This method of detection was shown to be rapid, species specific, and unaffected by strain variation or the presence of other organisms. Detection of M. meleagridis in clinical samples by PCR was achieved and later confirmed by culture and growth inhibition. Definitive identification by culture and growth inhibition required up to 3 weeks, whereas positive results from PCR testing were obtained within a day and negative samples were confirmed within 4 days.

摘要

基于聚合酶链反应（PCR）的诊断测试使用针对16S核糖体RNA的寡核苷酸，用于特异性检测火鸡病原体火鸡支原体和衣阿华支原体。这种检测方法被证明是快速、种属特异性的，不受菌株变异或其他生物体存在的影响。通过PCR在临床样本中检测到了火鸡支原体，随后通过培养和生长抑制进行了确认。通过培养和生长抑制进行明确鉴定需要长达3周的时间，而PCR检测在一天内即可获得阳性结果，阴性样本在4天内得到确认。

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