Enolase and the arsonomethyl analogue of 2-phosphoglycerate.
作者信息
Chawla S, Dixon H B
机构信息
Department of Biochemistry, University of Cambridge, UK.
出版信息
J Enzyme Inhib. 1995;8(4):255-9. doi: 10.3109/14756369509020132.
PMID:7542322
Abstract
(RS)-3-Arsono-2-(hydroxymethyl)propionic acid was synthesized by the action of alkaline arsenite on 3-bromo-2-(bromomethyl)propionic acid. It is a substrate for yeast enolase (EC 4.2.1.11) with a Km of 6.5 mM (for 2-phospho-D-glycerate Km = 0.08 mM). The catalytic constant of the enzyme with the arsonomethyl analogue is 230 times lower than with 2-phosphoglycerate.
摘要
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