Rahmoune H, Shephard K L
Clore Laboratory for Life Sciences, University of Buckingham, UK.
Pulm Pharmacol. 1994 Aug;7(4):265-9. doi: 10.1006/pulp.1994.1030.
Isolated guinea-pig tracheae exposed to conditioned air mucosally and to circulating saline solutions serosally have been subjected to an osmotic gradient between saline and airway surface and to a range of drugs. Experiments were conducted to examine the mechanisms that regulate airway surface liquid during condensation and evaporation. Measurements were made of the depth of airway surface liquid, its sodium activity, the transepithelial potential difference, and of net water and sodium transport across the airway. Results suggest that osmotic gradients have profound effects on airway surface liquid but drugs that influence secretory activity (methacholine, substance P) or ion transport activity (amiloride, bumetanide, oubain) do not.
将豚鼠离体气管黏膜暴露于经处理的空气中，浆膜暴露于循环盐溶液中，使其处于盐水与气道表面之间的渗透梯度以及一系列药物作用之下。进行实验以研究在冷凝和蒸发过程中调节气道表面液体的机制。测量了气道表面液体的深度、其钠活性、跨上皮电位差以及气道内水和钠的净转运。结果表明，渗透梯度对气道表面液体有深远影响，但影响分泌活性的药物（乙酰甲胆碱、P物质）或离子转运活性的药物（氨氯吡咪、布美他尼、哇巴因）则无此作用。
