Sagach V F, Tkachenko M N
Department of Physiology of Blood Circulation, A.A. Bogomolets Institute of Physiology, National Academy of Sciences, Kiev, Ukraine.
Pol J Pharmacol. 1994 Nov-Dec;46(6):609-14.
Reactive hyperemia (RH) was produced after short-term (5-30 s) cessation of blood flow in experiments on anesthetized dogs following catheterization of the circumflex branch of the left coronary artery (with the chest intact) autoperfused with blood from the subclavian artery. The increase of the coronary blood flow observed after occlusion was shown to depend strictly on its duration. Deendothelization of coronary vessels decreased the RH significantly. In coronary bed the infusion of L-arginine was accompanied by an increase of RH and endothelium-dependent acetylcholine-induced vasodilation. L-arginine produced its effect only when the endothelium was intact, whereas NO synthase inhibition substantially decreased the RH and vasodilating response to intracoronary administration of acetylcholine. It was concluded that the RH was endothelium-dependent and that nitric oxide of endothelial origin was the active humoral component of this reaction.
在对麻醉犬进行的实验中，通过在左冠状动脉回旋支插管（胸部完整）后，短暂（5 - 30秒）停止血流来产生反应性充血（RH），该冠状动脉由锁骨下动脉自灌注血液。结果显示，闭塞后观察到的冠状动脉血流量增加严格取决于其持续时间。冠状动脉血管去内皮化显著降低了反应性充血。在冠状动脉床中，输注L - 精氨酸伴随着反应性充血的增加以及内皮依赖性乙酰胆碱诱导的血管舒张。L - 精氨酸仅在内皮完整时才产生作用，而一氧化氮合酶抑制则显著降低了反应性充血以及对冠状动脉内给予乙酰胆碱的血管舒张反应。得出的结论是，反应性充血是内皮依赖性的，并且内皮来源的一氧化氮是该反应的活性体液成分。
