Bargatze R F, Jutila M A, Butcher E C
Department of Pathology, Stanford University School of Medicine, California 94305, USA.
Immunity. 1995 Jul;3(1):99-108. doi: 10.1016/1074-7613(95)90162-0.
Circulating lymphocytes home to the mucosal lymphoid organs, Peyer's patches (PP), through high endothelial venules (HEV). In situ analyses revealed that transfused lymph node cells (LNCs) interact with PP-HEV in a series of overlapping adhesion events: L-selectin (CD62L) > alpha 4 beta 7 initiates interaction, L-selectin and alpha 4 beta 7 both participate in rolling, and G alpha i-linked activation triggers arrest that requires both alpha 4 beta 7 and LFA-1. alpha 4 beta 7 dramatically reduces rolling velocity, and appears to be required for engagement of LFA-1. In contrast with resting LNC, preactivated LNC or alpha 4 beta 7hi lymphoma cells require only alpha 4 beta 7 for arrest in PP-HEV. The predominant PP-HEV ligand for alpha 4 beta 7 but also apparently for L-selectin is the mucosal addressin MAd-CAM-1. These results validate the concept of multimolecular adhesion/decision cascades in physiologic lymphocyte-endothelial recognition, define a novel role for alpha 4 integrins as a "bridge" between selectin and beta 2 integrin-dependent events, and reemphasize the potential for direct adhesion through preactivated alpha 4 integrins alone.
循环淋巴细胞通过高内皮微静脉（HEV）归巢至黏膜淋巴器官——派尔集合淋巴结（PP）。原位分析显示，输注的淋巴结细胞（LNC）在一系列重叠的黏附事件中与PP-HEV相互作用：L-选择素（CD62L）>α4β7启动相互作用，L-选择素和α4β7均参与滚动，而Gαi偶联的激活触发停滞，这需要α4β7和淋巴细胞功能相关抗原-1（LFA-1）两者。α4β7显著降低滚动速度，并且似乎是LFA-1结合所必需的。与静息LNC不同，预激活的LNC或α4β7高表达的淋巴瘤细胞在PP-HEV中停滞仅需要α4β7。α4β7以及显然还有L-选择素的主要PP-HEV配体是黏膜定居素细胞黏附分子-1（MAd-CAM-1）。这些结果验证了生理淋巴细胞-内皮识别中多分子黏附/决策级联的概念，定义了α4整合素作为选择素和β2整合素依赖性事件之间“桥梁”的新作用，并再次强调了仅通过预激活的α4整合素直接黏附的可能性。
