Bourget I, Di Berardino W, Breittmayer J P, Grenier-Brossette N, Plana-Prades M, Bonnefoy J Y, Cousin J L
Laboratoire d'Immunologie Cellulaire et Moléculaire INSERM U364, Faculté de Médecine, Pasteur, Nice, France.
Eur J Immunol. 1995 Jul;25(7):1872-6. doi: 10.1002/eji.1830250712.
CD20 monoclonal antibody (mAb) B1 is known to inhibit B cell proliferation. We show that B1 reduced both anti-mu + interleukin-4 (IL-4)-induced DNA synthesis and the concomitant expression of CD23 at the surface of human tonsillar B cells. B1 mAb had no effect on CD23 mRNA levels. The disappearance of CD23 molecule from the surface correlates with an increase of soluble CD23 fragments in the culture medium, indicating that CD20 mAb B1 stimulated the cleavage of the molecule. B1 also inhibits IgE production by peripheral blood mononuclear cells cultured in the presence of IL-4. Suppression of IgE synthesis and enhancement of CD23 cleavage are concomitant but appear not to be functionally related.
已知CD20单克隆抗体（mAb）B1可抑制B细胞增殖。我们发现，B1可降低抗μ + 白细胞介素-4（IL-4）诱导的DNA合成以及人扁桃体B细胞表面CD23的伴随表达。B1单克隆抗体对CD23 mRNA水平没有影响。CD23分子从表面消失与培养基中可溶性CD23片段的增加相关，表明CD20单克隆抗体B1刺激了该分子的裂解。B1还可抑制在IL-4存在下培养的外周血单核细胞产生IgE。IgE合成的抑制和CD23裂解的增强是同时发生的，但似乎在功能上没有关联。
