Spudich A, Braunstein D
Department of Molecular and Cellular Physiology, Stanford University, CA 94305, USA.
Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):6976-80. doi: 10.1073/pnas.92.15.6976.
Scanning force microscopy was used to image rat basophilic leukemia (RBL-2H3) cell surfaces under different stimulation conditions that either permit or inhibit secretion. Cross-linking the surface IgE receptors with dinitrophenol-conjugated bovine serum albumin initiates secretion in RBL cells with concomitant spreading of the cell body. Structures at the cell surface approximately 1.5 microns in diameter relate to secretion both spatially and temporally. The position of these surface pits and their sizes suggest that they may be related to the dense-core granules positioned along the cytoskeletal filaments in detergent-extracted, unactivated RBL cell processes. Topographic scanning force microscopy images of RBL cell surfaces at 2, 5, and 35 min after activation show that these structures persist and change in cross-sectional profile with time after activation. These structures may be related to the membrane retrieval mechanism of cells after intense stimulation.
利用扫描力显微镜对大鼠嗜碱性白血病（RBL - 2H3）细胞在允许或抑制分泌的不同刺激条件下的细胞表面进行成像。用二硝基苯酚偶联的牛血清白蛋白交联表面IgE受体可引发RBL细胞分泌，并伴随细胞体铺展。细胞表面直径约1.5微米的结构在空间和时间上均与分泌有关。这些表面凹陷的位置及其大小表明，它们可能与去污剂提取的未激活RBL细胞突起中沿细胞骨架丝排列的致密核心颗粒有关。激活后2分钟、5分钟和35分钟时RBL细胞表面的形貌扫描力显微镜图像显示，这些结构持续存在，并在激活后的时间里横截面轮廓发生变化。这些结构可能与细胞在强烈刺激后的膜回收机制有关。
