Xu W, Charles I G, Liu L, Koni P A, Moncada S, Emson P
MRC Molecular Neuroscience Group, Department of Neurobiology, Babraham Institute, Cambridge, UK.
Biochem Biophys Res Commun. 1995 Jul 17;212(2):466-72. doi: 10.1006/bbrc.1995.1993.
In previous studies, we found multiple copies of inducible nitric oxide synthase gene (NOS2)-like sequences in the human genome and mapped them to the pericentric region of chromosome 17. Here, we describe the cloning and sequencing of exon22 regions from three of these NOS2-like sequences. We have also mapped another NOS2-like sequence to human chromosome 14. Since there are multiple NOS2-like sequences present in the human genome, we have also carried out Zoo Blot hybridisation analysis using a NOS2 cDNA probe. Our data suggest that duplication of NOS2-like sequences occurred very recently in primate evolution.
在先前的研究中，我们在人类基因组中发现了多个诱导型一氧化氮合酶基因（NOS2）样序列的拷贝，并将它们定位到17号染色体的着丝粒区域。在此，我们描述了其中三个NOS2样序列的外显子22区域的克隆和测序。我们还将另一个NOS2样序列定位到人类14号染色体。由于人类基因组中存在多个NOS2样序列，我们还使用NOS2 cDNA探针进行了人-动物杂交分析。我们的数据表明，NOS2样序列的复制在灵长类动物进化中是最近才发生的。
