Hamasaki K, Mimura T, Furuya H, Morino N, Yamazaki T, Komuro I, Yazaki Y, Nojima Y
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1995 Jul 17;212(2):544-9. doi: 10.1006/bbrc.1995.2004.
Mechanical loading of cultured mesangial cells has been shown to induce both increased production of matrix proteins and cell proliferation. This in vitro phenomenon has been implicated in the progression of glomerular sclerosis observed in a variety of glomerular diseases. However, it is not yet known how cells sense mechanical stress and transfer this sense into biochemical and biological events. In this study, we show that mesangial stretching rapidly stimulates tyrosine phosphorylation of non-receptor tyrosine kinase pp125FAK. Since pp125FAK is a major constituent of focal adhesions, our findings suggest that the focal adhesion may be a site where mechanical forces are translated into biochemical events, and that pp125FAK may play an important role in this signaling cascade.
体外培养的系膜细胞在机械负荷下，已被证实会导致基质蛋白生成增加和细胞增殖。这一体外现象与多种肾小球疾病中观察到的肾小球硬化进展有关。然而，细胞如何感知机械应力并将这种感知转化为生化和生物学事件尚不清楚。在本研究中，我们发现系膜细胞拉伸可迅速刺激非受体酪氨酸激酶pp125FAK的酪氨酸磷酸化。由于pp125FAK是粘着斑的主要组成部分，我们的研究结果表明粘着斑可能是机械力转化为生化事件的部位，并且pp125FAK可能在这一信号级联反应中发挥重要作用。
