Yang Y, Huang L, Pon R T, Lown J W
University Core DNA Services, Faculty of Medicine, University of Calgary, Alberta, Canada.
Biochem Biophys Res Commun. 1995 Jul 26;212(3):995-1000. doi: 10.1006/bbrc.1995.2068.
The interaction between the cobalt complex of a bleomycin functional model and d(CGCAATTGCG)2 was determined by 2D NMR methods. The intermolecular NOE cross peaks between ligand protons and the DNA minor groove protons suggest that the cobalt complex of AMPHIS-NET binds in the minor groove of DNA at the central AATT site. Also, the NOEs interactions of H8 pyridine proton and H2 imidazole proton in the metal-binding domain with H4' sugar proton of C9 and H4' sugar proton of A15 demonstrate that the sixth ligand site of cobalt complex, which is occupied by a solvent molecule, faces towards the minor groove of DNA. This binding model is in accord with the observed non-diffusion DNA cleavage by the metal-complexes of the bleomycin functional model compounds.
通过二维核磁共振方法确定了博来霉素功能模型的钴配合物与d(CGCAATTGCG)2之间的相互作用。配体质子与DNA小沟质子之间的分子间核Overhauser效应（NOE）交叉峰表明，AMPHIS-NET的钴配合物在DNA中央AATT位点的小沟中结合。此外，金属结合域中H8吡啶质子和H2咪唑质子与C9的H4'糖质子和A15的H4'糖质子之间的NOE相互作用表明，被溶剂分子占据的钴配合物的第六个配位点朝向DNA小沟。这种结合模式与博来霉素功能模型化合物的金属配合物观察到的非扩散性DNA切割一致。
