Flores-Herrera O, Pardo J P, Espinosa-García M T, Martínez F
Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma, México, D.F., México.
Biochem Mol Biol Int. 1995 Apr;35(4):793-801.
The kinetic characteristics of the transport of Ca2+ in the human term placental mitochondria previously depleted of their intramitochondrial calcium, was studied. Maximal Ca2+ uptake was observed in the presence of inorganic phosphate and ADP. Ca2+ transport showed a Michaelis-Menten behavior, with a Vmax of 61 nmol mg-1 min-1 and a Km of 118 microM. Ruthenium red inhibited mitochondrial Ca2+ uptake. Under optimal conditions of Ca2+ uptake, the cation inhibited progesterone synthesis in placental mitochondria. The kinetic parameters of Ca2+ transport agree with the physiological role of mitochondria in the human placenta.
对先前已耗尽线粒体内钙的人足月胎盘线粒体中Ca2+转运的动力学特性进行了研究。在无机磷酸盐和ADP存在的情况下观察到最大Ca2+摄取。Ca2+转运表现出米氏动力学行为，Vmax为61 nmol mg-1 min-1，Km为118 microM。钌红抑制线粒体Ca2+摄取。在Ca2+摄取的最佳条件下，该阳离子抑制胎盘线粒体中的孕酮合成。Ca2+转运的动力学参数与线粒体在人胎盘中的生理作用相符。
