Park J G, Lee J H, Kang M S, Park K J, Jeon Y M, Lee H J, Kwon H S, Park H S, Yeo K S, Lee K U
Laboratory of Cell Biology, Korean Cell Line Research Foundation, Seoul.
Int J Cancer. 1995 Jul 28;62(3):276-82. doi: 10.1002/ijc.2910620308.
We characterized 8 human hepatocellular-carcinoma cell lines established from the primary tumors of Korean patients. All lines showed substrate adherence and one line from anaplastic tumor also grew as floating aggregates. Most cultured cells maintained many morphological characteristics of the original tumors from which they were derived. Doubling times varied from 34 to 72 hr. All lines showed relatively high viability and were not contaminated with Mycoplasma or bacteria. All lines showed aneuploidy and were proven to be unique by DNA fingerprinting analysis. Hepatitis-B-virus (HBV) DNA was integrated in the genomes of all lines. Two of the cell lines (SNU-354, SNU-368) showed expression of HBV and HBVx (HBx) transcripts. SNU-354 strongly expressed albumin, and SNU-368 expressed transferrin and insulin-like growth factor II. No lines produced alpha-fetoprotein at the RNA and protein level. These cell lines represent useful tools for in vitro studies related to hepatocellular carcinoma.
我们对从韩国患者原发性肿瘤中建立的8种人肝癌细胞系进行了特征分析。所有细胞系均表现出贴壁生长，其中一种来自间变性肿瘤的细胞系还能以悬浮聚集体的形式生长。大多数培养细胞保留了其来源的原始肿瘤的许多形态特征。倍增时间从34小时到72小时不等。所有细胞系均表现出相对较高的活力，且未被支原体或细菌污染。所有细胞系均显示非整倍体，经DNA指纹分析证明是独特的。乙型肝炎病毒（HBV）DNA整合在所有细胞系的基因组中。其中两个细胞系（SNU-354、SNU-368）显示出HBV和HBVx（HBx）转录本的表达。SNU-354强烈表达白蛋白，SNU-368表达转铁蛋白和胰岛素样生长因子II。没有细胞系在RNA和蛋白质水平产生甲胎蛋白。这些细胞系是用于肝细胞癌相关体外研究的有用工具。
