Ascoli V, Scalzo C C, Facciolo F, Nardi F
Department of Experimental Medicine, La Sapienza University, Rome, Italy.
Acta Cytol. 1995 Jul-Aug;39(4):613-22.
Expression of the platelet-derived growth factor receptor (PDGFR) was detected by immunocytochemistry in normal and malignant mesothelial cells from 14 benign effusions and 22 mesotheliomas. Two well-characterized antireceptor monoclonal antibodies to the PDGFR alpha-subunit (PR292) and beta-subunit (PR7212) were used. PDGFR alpha-subunit outlined cell membranes intensely in nonneoplastic mesothelial cells, whereas it was focal in mesothelioma and limited to a few cases only. PDGFR beta-subunit was weakly expressed in the cytoplasm of normal mesothelium; in contrast, malignant mesothelial cells showed strong cytoplasmic staining. Our results show that normal mesothelium may be responsive to PDGF by the predominant expression of PDGFR-alpha and less by PDGFR-beta and indicate the presence of growth-stimulation loops in mesothelioma through PDGF/PDGFR-beta interaction. Also, scattered staining for the PDGFR alpha-subunit suggests that a PDGF-A chain/PDGFR-alpha interaction may also be active in mesothelioma cells growing in suspension.
采用免疫细胞化学方法检测了来自14例良性积液和22例间皮瘤的正常和恶性间皮细胞中血小板衍生生长因子受体（PDGFR）的表达。使用了两种针对PDGFRα亚基（PR292）和β亚基（PR7212）的特性明确的抗受体单克隆抗体。PDGFRα亚基在非肿瘤性间皮细胞中强烈勾勒出细胞膜，而在间皮瘤中呈局灶性，仅在少数病例中出现。PDGFRβ亚基在正常间皮的细胞质中弱表达；相比之下，恶性间皮细胞显示出强烈的细胞质染色。我们的结果表明，正常间皮可能通过主要表达PDGFR-α且较少表达PDGFR-β来对PDGF产生反应，并表明通过PDGF/PDGFR-β相互作用在间皮瘤中存在生长刺激环。此外，PDGFRα亚基的散在染色表明，PDGF-A链/PDGFR-α相互作用在悬浮生长的间皮瘤细胞中也可能活跃。
