Knefati M, Somogyi C, Kapás L, Bourcier T, Krueger J M
Department of Neurology, University of Tennessee, Memphis 38163, USA.
Am J Physiol. 1995 Jul;269(1 Pt 2):R87-91. doi: 10.1152/ajpregu.1995.269.1.R87.
Acidic fibroblast growth factor (FGF) and basic FGF belong to a growth factor family. Interleukin-1, another member of that family, is involved in sleep regulation. FGFs and interleukin-1 share structural and functional features. We therefore determined whether acidic FGF and basic FGF were somnogenic. Male New Zealand White rabbits were provided with electroencephalographic (EEG) electrodes, a brain thermistor, and a lateral intracerebroventricular (icv) cannula. The animals were injected icv with isotonic NaCl (control) and on separate days with one of three doses of acidic or basic FGF (0.01, 0.1, or 1.0 micrograms) or with heat-treated acidic FGF (1.0 micrograms). The EEG, brain temperature, and motor activity were recorded for 23 h. The biological activity of basic FGF was determined in vitro by its ability to induce DNA synthesis in rat aortic smooth muscle cells. Acidic FGF induced prolonged dose-related increases in non-rapid eye movement sleep beginning in the 1st postinjection h and continuing for 12-23 h after the treatment. Acidic FGF also induced fevers of approximately 1 degree C after the 1.0 micrograms dose. Both activities of acidic FGF were lost after heat treatment. In contrast, basic FGF lacked somnogenic and pyrogenic activity, although it did induce DNA synthesis. Current results suggest that acidic FGF is part of the complex cytokine network in brain involved in sleep regulation.
酸性成纤维细胞生长因子（FGF）和碱性FGF属于生长因子家族。白细胞介素-1是该家族的另一个成员，参与睡眠调节。FGFs和白细胞介素-1具有结构和功能特征。因此，我们确定酸性FGF和碱性FGF是否具有促睡眠作用。给雄性新西兰白兔植入脑电图（EEG）电极、脑热敏电阻和侧脑室插管。动物经侧脑室注射等渗氯化钠（对照），并在不同日期分别注射三种剂量之一的酸性或碱性FGF（0.01、0.1或1.0微克）或经热处理的酸性FGF（1.0微克）。记录EEG、脑温和运动活动23小时。通过碱性FGF诱导大鼠主动脉平滑肌细胞DNA合成的能力在体外测定其生物活性。酸性FGF在注射后第1小时开始诱导非快速眼动睡眠的剂量相关延长增加，并在治疗后持续12 - 23小时。酸性FGF在1.0微克剂量后还诱导约1摄氏度的发热。酸性FGF的这两种活性在热处理后丧失。相比之下，碱性FGF缺乏促睡眠和致热活性，尽管它确实能诱导DNA合成。目前的结果表明，酸性FGF是参与睡眠调节的脑内复杂细胞因子网络的一部分。
